Cell Biology Antibodies 4

Anti-HRH2 Antibody (CAB14170)

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SKU:
CAB14170
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-HRH2 Antibody
Antibody SKU:CAB14170
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human HRH2 (NP_001124527.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, A-431, Mouse skeletal muscle
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 150-250 of human HRH2 (NP_001124527.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SFLS IHLG WNSR NETS KGNH TTSK CKVQ VNEV YGLV DGLV TFYL PLLI MCIT YYRI FKVA RDQA KRIN HISS WKAA TIRE HKAT VTLA AVMG AFII CWFP Y
Gene ID:3274
Uniprot:P25021
Cellular Location:Cell membrane, Multi-pass membrane protein
Calculated MW:40kDa/44kDa
Observed MW:38kDa
Synonyms:HRH2, H2R
Background:Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:H2R: The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: integral to plasma membrane; plasma membrane

Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; immune response

NCBI Summary:Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. Histamine receptor H2 belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and stimulates gastric acid secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:P25021
NCBI GenInfo Identifier:123120
NCBI Gene ID:3274
NCBI Accession:P25021.1
UniProt Secondary Accession:P25021,Q14464, Q7Z5R9, B5BUP7,
UniProt Related Accession:P25021
Molecular Weight:44,541 Da
NCBI Full Name:Histamine H2 receptor
NCBI Synonym Full Names:histamine receptor H2
NCBI Official Symbol:HRH2  
NCBI Official Synonym Symbols:H2R  
NCBI Protein Information:histamine H2 receptor
UniProt Protein Name:Histamine H2 receptor
UniProt Synonym Protein Names:Gastric receptor I
Protein Family:Histamine H2 receptor
UniProt Gene Name:HRH2  
UniProt Entry Name:HRH2_HUMAN
Anti-HRH2 Antibody (CAB14170)Western blot analysis of extracts of various cell lines, using HRH2 antibody (CAB14170) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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