Antibody Name:	Anti-HRAS Antibody
Antibody SKU:	CAB12212
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human HRAS (NP_005334.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human HRAS (NP_005334.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTEY KLVV VGAG GVGK SALT IQLI QNHF VDEY DPTI EDSY RKQV VIDG ETCL LDIL DTAG QEEY SAMR DQYM RTGE GFLC VFAI NNTK SFED IHQY REQI KRVK DSDD VPMV LVGN KCDL AART VESR QAQD LARS YGIP YIET SAKT RQGV EDAF YTLV REIR QHKL RKLN PPDE SGPG CMSC KCVL S
Gene ID:	3265
Uniprot:	P01112
Cellular Location:	Cell membrane, Cytoplasm, Cytoplasmic side, Golgi apparatus, Golgi apparatus membrane, Lipid-anchor, Lipid-anchor, Nucleus, perinuclear region
Calculated MW:	18kDa/21kDa
Observed MW:	19kDa

Synonyms:

C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras, GTPase HRAS, HRAS

Background:

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

UniProt Protein Function:	Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151).
NCBI Summary:	This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P01112
NCBI GenInfo Identifier:	131869
NCBI Gene ID:	3265
NCBI Accession:	P01112.1
UniProt Secondary Accession:	P01112,Q14080, Q6FHV9, Q9BR65, Q9UCE2, B5BUA0,
UniProt Related Accession:	P01112
Molecular Weight:	21kDa
NCBI Full Name:	GTPase HRas
NCBI Synonym Full Names:	HRas proto-oncogene, GTPase
NCBI Official Symbol:	HRAS
NCBI Official Synonym Symbols:	CTLO; HAMSV; HRAS1; RASH1; p21ras; C-H-RAS; H-RASIDX; C-BAS/HAS; C-HA-RAS1
NCBI Protein Information:	GTPase HRas
UniProt Protein Name:	GTPase HRas
UniProt Synonym Protein Names:	H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras
Protein Family:	HRAS-like suppressor
UniProt Gene Name:	HRAS

	Western blot analysis of extracts of mouse brain, using HRAS Antibody (CAB12212) at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using HRAS antibody (CAB12212) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.