Name: Anti-HPSE2 Antibody (CAB8248)
Brand: Cell Biology Antibodies 12
SKU: CAB8248
Availability: OutOfStock

Antibody Name:	HPSE2 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8248
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 42-242 of human HPSE2 (NP_068600.4).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, 22Rv1, HeLa, SKOV3, Mouse brain, Mouse small intestine, Mouse testis, Mouse uterus, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 42-242 of human HPSE2 (NP_068600.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GDRR PLPV DRAA GLKE KTLI LLDV STKN PVRT VNEN FLSL QLDP SIIH DGWL DFLS SKRL VTLA RGLS PAFL RFGG KRTD FLQF QNLR NPAK SRGG PGPD YYLK NYED DIVR SDVA LDKQ KGCK IAQH PDVM LELQ REKA AQMH LVLL KEQF SNTY SNLI LTAR SLDK LYNF ADCS GLHL IFAL NALR RNPN NSWN SSSA L
Gene ID:	60495
Uniprot:	Q8WWQ2
Cellular Location:	Secreted, extracellular matrix, extracellular space
Calculated MW:	53kDa/60kDa/61kDa/66kDa
Observed MW:	72kDa

Synonyms:	HPSE2, HPA2, HPR2, UFS, UFS1
Background:	This gene encodes a heparanase enzyme. The encoded protein is a endoglycosidase that degrades heparin sulfate proteoglycans located on the extracellular matrix and cell surface. This protein may be involved in biological processes involving remodeling of the extracellular matrix including angiogenesis and tumor progression. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	HPSE2: Binds heparin and heparan sulfate with high affinity, but lacks heparanase activity. Inhibits HPSE, possibly by competing for its substrates (in vitro). Defects in HPSE2 are the cause of urofacial syndrome (UFS). A rare autosomal recessive characterized by facial grimacing when attempting to smile and failure of the urinary bladder to void completely despite a lack of anatomical bladder outflow obstruction or overt neurological damage. Affected individuals often have reflux of infected urine from the bladder to the upper renal tract, with a risk of kidney damage and renal failure. Belongs to the glycosyl hydrolase 79 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.2.-.-; Hydrolase; Glycan Metabolism - glycosaminoglycan degradation Chromosomal Location of Human Ortholog: 10q23-q24 Cellular Component: proteinaceous extracellular matrix; plasma membrane; intracellular Molecular Function:heparan sulfate proteoglycan binding; heparanase activity Biological Process: glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis Disease: Urofacial Syndrome 1
NCBI Summary:	This gene encodes a heparanase enzyme. The encoded protein is a endoglycosidase that degrades heparin sulfate proteoglycans located on the extracellular matrix and cell surface. This protein may be involved in biological processes involving remodeling of the extracellular matrix including angiogenesis and tumor progression. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
UniProt Code:	Q8WWQ2
NCBI GenInfo Identifier:	261878501
NCBI Gene ID:	60495
NCBI Accession:	NP_068600
UniProt Secondary Accession:	Q8WWQ2,Q5VUH4, Q5VUH5, Q5VUH6, Q8WWQ1, Q9HB37, Q9HB38 Q9HB39,
UniProt Related Accession:	Q8WWQ2
Molecular Weight:	53,917 Da
NCBI Full Name:	inactive heparanase-2 isoform 1
NCBI Synonym Full Names:	heparanase 2 (inactive)
NCBI Official Symbol:	HPSE2
NCBI Official Synonym Symbols:	UFS; HPA2; HPR2; UFS1
NCBI Protein Information:	inactive heparanase-2
UniProt Protein Name:	Inactive heparanase-2
Protein Family:	Histone acetyltransferase
UniProt Gene Name:	HPSE2
UniProt Entry Name:	HPSE2_HUMAN

	Western blot analysis of extracts of various cell lines, using HPSE2 antibody (CAB8248) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human prostate using HPSE2 antibody (CAB8248) at dilution of 1:100 (40x lens).