Antibody Name:	Anti-HPRT1 Antibody [KO Validated]
Antibody SKU:	CAB5692
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human HPRT1 (NP_000185.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF-7, HL-60, SW480, 293T, HeLa, Mouse brain, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human HPRT1 (NP_000185.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATR SPGV VISD DEPG YDLD LFCI PNHY AEDL ERVF IPHG LIMD RTER LARD VMKE MGGH HIVA LCVL KGGY KFFA DLLD YIKA LNRN SDRS IPMT VDFI RLKS YCND QSTG DIKV IGGD DLST LTGK NVLI VEDI IDTG KTMQ TLLS LVRQ YNPK MVKV ASLL VKRT PRSV GYKP DFVG FEIP DKFV VGYA LDYN EYFR DLNH VCVI SETG KAKY KA
Gene ID:	3251
Uniprot:	P00492
Cellular Location:	Cytoplasm
Calculated MW:	24kDa
Observed MW:	27kDa

Synonyms:

HPRT1, HGPRT, HPRT

Background:

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

UniProt Protein Function:	HPRT1: Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5- phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. Defects in HPRT1 are the cause of Lesch-Nyhan syndrome (LNS). LNS is characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation. Defects in HPRT1 are the cause of gout HPRT-related (GOUT-HPRT); also known as HPRT-related gout or Kelley-Seegmiller syndrome. Gout is characterized by partial enzyme activity and hyperuricemia. Belongs to the purine/pyrimidine phosphoribosyltransferase family.
UniProt Protein Details:	Protein type:EC 2.4.2.8; Xenobiotic Metabolism - drug metabolism - other enzymes; Transferase; Nucleotide Metabolism - purine; Cell development/differentiation Chromosomal Location of Human Ortholog: Xq26.1 Cellular Component: cytoplasm; cytosol Molecular Function:protein binding; protein homodimerization activity; hypoxanthine phosphoribosyltransferase activity; magnesium ion binding; nucleotide binding Biological Process: grooming behavior; lymphocyte proliferation; hypoxanthine metabolic process; IMP salvage; striatum development; nucleobase, nucleoside and nucleotide metabolic process; GMP salvage; dendrite morphogenesis; cytolysis; response to amphetamine; locomotory behavior; adenine salvage; purine nucleotide biosynthetic process; dopamine metabolic process; purine base metabolic process; purine salvage; cerebral cortex neuron differentiation; protein homotetramerization; positive regulation of dopamine metabolic process; GMP catabolic process; IMP metabolic process; hypoxanthine salvage; purine ribonucleoside salvage; central nervous system neuron development; guanine salvage Disease: Kelley-seegmiller Syndrome; Lesch-nyhan Syndrome
NCBI Summary:	The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]
UniProt Code:	P00492
NCBI GenInfo Identifier:	123497
NCBI Gene ID:	3251
NCBI Accession:	P00492.2
UniProt Secondary Accession:	P00492,A6NHF0, B2R8M9,
UniProt Related Accession:	P00492
Molecular Weight:	218
NCBI Full Name:	Hypoxanthine-guanine phosphoribosyltransferase
NCBI Synonym Full Names:	hypoxanthine phosphoribosyltransferase 1
NCBI Official Symbol:	HPRT1
NCBI Official Synonym Symbols:	HPRT; HGPRT
NCBI Protein Information:	hypoxanthine-guanine phosphoribosyltransferase; HGPRTase
UniProt Protein Name:	Hypoxanthine-guanine phosphoribosyltransferase
Protein Family:	Hypoxanthine-guanine phosphoribosyltransferase
UniProt Gene Name:	HPRT1
UniProt Entry Name:	HPRT_HUMAN

	Western blot analysis of extracts of various cell lines, using HPRT1 antibody (CAB5692) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Western blot analysis of extracts from normal (control) and HPRT1 knockout (KO) 293T cells, using HPRT1 antibody (CAB5692) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of U2OS cells using HPRT1 antibody (CAB5692). Blue: DAPI for nuclear staining.