Name: Anti-HPD Antibody (CAB6505)
Brand: Metabolism Antibodies 2
SKU: CAB6505
Availability: OutOfStock

Antibody Name:	Anti-HPD Antibody
Antibody SKU:	CAB6505
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human HPD (NP_002141.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, Mouse liver, Mouse kidney, Rat liver, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human HPD (NP_002141.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTTY SDKG AKPE RGRF LHFH SVTF WVGN AKQA ASFY CSKM GFEP LAYR GLET GSRE VVSH VIKQ GKIV FVLS SALN PWNK EMGD HLVK HGDG VKDI AFEV EDCD YIVQ KARE RGAK IMRE PWVE QDKF GKVK FAVL QTYG DTTH TLVE KMNY IGQF LPGY EAPA FMDP LLPK LPKC SLEM IDHI VGNQ PDQE MVSA SEWY LKNL QFHR FWSV DDTQ VHTE YSSL RSIV VANY EESI KMPI NEPA PGKK KSQI QEYV DYNG GAGV QHIA LKTE DIIT AIRH LRER GLEF LSVP STYY KQLR EKLK TAKI KVKE NIDA LEEL KILV DYDE KGYL LQIF TKPV QDRP TLFL EVIQ RHNH QGFG AGNF NSLF KAFE EEQN LRGN LTNM ETNG VVPG M
Gene ID:	3242
Uniprot:	P32754
Cellular Location:
Calculated MW:	40kDa/44kDa
Observed MW:	45kDa

Synonyms:	HPD, 4-HPPD, 4HPPD, GLOD3, HPPDASE, PPD
Background:	The protein encoded by this gene is an enzyme in the catabolic pathway of tyrosine. The encoded protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate. Defects in this gene are a cause of tyrosinemia type 3 (TYRO3) and hawkinsinuria (HAWK). Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	HPD: Key enzyme in the degradation of tyrosine. Defects in HPD are the cause of tyrosinemia type 3 (TYRO3). TYRO3 is an inborn error of metabolism characterized by elevations of tyrosine in the blood and urine, seizures and mild mental retardation. Defects in HPD are a cause of hawkinsinuria (HAWK). HAWK is an inborn error of tyrosine metabolism characterized by failure to thrive, persistent metabolic acidosis, fine and sparse hair, and excretion of the unusual cyclic amino acid metabolite, hawkinsin, in the urine. Belongs to the 4HPPD family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 1.13.11.27; Cofactor and Vitamin Metabolism - ubiquinone and other terpenoid-quinone biosynthesis; Amino Acid Metabolism - phenylalanine; Oxidoreductase; Amino Acid Metabolism - tyrosine Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: cytosol Molecular Function:4-hydroxyphenylpyruvate dioxygenase activity; metal ion binding Biological Process: L-phenylalanine catabolic process; tyrosine catabolic process Disease: Hawkinsinuria; Tyrosinemia, Type Iii
NCBI Summary:	The protein encoded by this gene is an enzyme in the catabolic pathway of tyrosine. The encoded protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate. Defects in this gene are a cause of tyrosinemia type 3 (TYRO3) and hawkinsinuria (HAWK). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
UniProt Code:	P32754
NCBI GenInfo Identifier:	417144
NCBI Gene ID:	3242
NCBI Accession:	P32754.2
UniProt Secondary Accession:	P32754,Q13234, A8K461, B3KQ63,
UniProt Related Accession:	P32754
Molecular Weight:	393
NCBI Full Name:	4-hydroxyphenylpyruvate dioxygenase
NCBI Synonym Full Names:	4-hydroxyphenylpyruvate dioxygenase
NCBI Official Symbol:	HPD
NCBI Official Synonym Symbols:	PPD; 4HPPD; GLOD3; 4-HPPD; HPPDASE
NCBI Protein Information:	4-hydroxyphenylpyruvate dioxygenase; glyoxalase domain containing 3; 4-hydroxyphenylpyruvic acid oxidase
UniProt Protein Name:	4-hydroxyphenylpyruvate dioxygenase
UniProt Synonym Protein Names:	4-hydroxyphenylpyruvic acid oxidase; 4HPPD; HPD; HPPDase
UniProt Gene Name:	HPD
UniProt Entry Name:	HPPD_HUMAN

	Western blot analysis of extracts of various cell lines, using HPD antibody (CAB6505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat kidney using HPD antibody (CAB6505) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using HPD antibody (CAB6505) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using HPD antibody (CAB6505). Blue: DAPI for nuclear staining.