Antibody Name:	Anti-HOXD10 Antibody
Antibody SKU:	CAB7686
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HOXD10 (NP_002139.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, Mouse heart, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HOXD10 (NP_002139.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSFP NSSP AANT FLVD SLIS ACRS DSFY SSSA SMYM PPPS ADMG TYGM QTCG LLPS LAKR EVNH QNMG MNVH PYIP QVDS WTDP NRSC RIEQ PVTQ QVPT CSFT TNIK EESN CCMY SDKR NKLI SAEV PSYQ RLVP ESCP VENP EVPV PGYF RLSQ TYAT GKTQ EYNN SPEG SSTV MLQL NPRG AAKP QLSA AQLQ MEKK MNEP VSGQ EPTK VSQV ESPE AKGG LPEE RSCL AEVS VSSP
Gene ID:	3236
Uniprot:	P28358
Cellular Location:	Nucleus
Calculated MW:	38kDa
Observed MW:	38kDa

Synonyms:

HOXD10, HOX4, HOX4D, HOX4E, Hox-4.4

Background:

This gene is a member of the Abd-B homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox D genes located on chromosome 2. The encoded nuclear protein functions as a sequence-specific transcription factor that is expressed in the developing limb buds and is involved in differentiation and limb development. Mutations in this gene have been associated with Wilm's tumor and congenital vertical talus (also known as 'rocker-bottom foot' deformity or congenital convex pes valgus) and/or a foot deformity resembling that seen in Charcot-Marie-Tooth disease.

UniProt Protein Function:	HOXD10: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Defects in HOXD10 are a cause of congenital vertical talus (CVT); also known as rocker-bottom foot deformity or congenital convex pes valgus. CVT is a dislocation of the talonavicular joint, with rigid dorsal dislocation of the navicular over the neck of the talus. This condition is usually associated with multiple other congenital deformities and only rarely is an isolated deformity. Belongs to the Abd-B homeobox family.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 2q31.1 Disease: Vertical Talus, Congenital
NCBI Summary:	This gene is a member of the Abd-B homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox D genes located on chromosome 2. The encoded nuclear protein functions as a sequence-specific transcription factor that is expressed in the developing limb buds and is involved in differentiation and limb development. Mutations in this gene have been associated with Wilm's tumor and congenital vertical talus (also known as "rocker-bottom foot" deformity or congenital convex pes valgus) and/or a foot deformity resembling that seen in Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008]
UniProt Code:	P28358
NCBI GenInfo Identifier:	143811403
NCBI Gene ID:	3236
NCBI Accession:	P28358.2
UniProt Secondary Accession:	P28358,Q6NT10,
UniProt Related Accession:	P28358
Molecular Weight:	38,411 Da
NCBI Full Name:	Homeobox protein Hox-D10
NCBI Synonym Full Names:	homeobox D10
NCBI Official Symbol:	HOXD10
NCBI Official Synonym Symbols:	HOX4; HOX4D; HOX4E; Hox-4.4
NCBI Protein Information:	homeobox protein Hox-D10
UniProt Protein Name:	Homeobox protein Hox-D10
UniProt Synonym Protein Names:	Homeobox protein Hox-4D; Homeobox protein Hox-4E
Protein Family:	Homeobox protein
UniProt Gene Name:	HOXD10
UniProt Entry Name:	HXD10_HUMAN

	Western blot analysis of extracts of various cell lines, using HOXD10 antibody (CAB7686) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using HOXD10 antibody (CAB7686) at dilution of 1:100. Blue: DAPI for nuclear staining.