Antibody Name:	Anti-HOXA11 Antibody
Antibody SKU:	CAB2976
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HOXA11 (NP_005514.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	SKOV3, HeLa, THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HOXA11 (NP_005514.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDFD ERGP CSSN MYLP SCTY YVSG PDFS SLPS FLPQ TPSS RPMT YSYS SNLP QVQP VREV TFRE YAIE PATK WHPR GNLA HCYS AEEL VHRD CLQA PSAA GVPG DVLA KSSA NVYH HPTP AVSS NFYS TVGR NGVL PQAF DQFF ETAY GTPE NLAS SDYP GDKS AEKG PPAA TATS AAAA
Gene ID:	3207
Uniprot:	P31270
Cellular Location:	Nucleus
Calculated MW:	34kDa
Observed MW:	35kDa

Synonyms:

HOXA11, HOX1, HOX1I, RUSAT1

Background:

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. This gene is involved in the regulation of uterine development and is required for female fertility. Mutations in this gene can cause radio-ulnar synostosis with amegakaryocytic thrombocytopenia.

UniProt Protein Function:	HOXA11: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Defects in HOXA11 are the cause of radioulnar synostosis with amegakaryocytic thrombocytopenia (RSAT). The syndrome consists of an unusual association of bone marrow failure and skeletal defects. Patients have the same skeletal defects, the proximal fusion of the radius and ulna, resulting in extremely limited pronation and supination of the forearm. Some patients have also symptomatic thrombocytopenia, with bruising and bleeding problems since birth, necessitating correction by bone marrow or umbilical-cord stem-cell transplantation. Belongs to the Abd-B homeobox family.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 7p15.2 Cellular Component: transcription factor complex; protein complex; nucleus Molecular Function:sequence-specific DNA binding Biological Process: embryonic forelimb morphogenesis; developmental growth; anatomical structure morphogenesis; transcription, DNA-dependent; multicellular organismal development; positive regulation of transcription, DNA-dependent; male gonad development; uterus development; anterior/posterior pattern formation; positive regulation of chondrocyte differentiation; dorsal/ventral pattern formation; ureteric bud branching; single fertilization; induction of an organ; mesodermal cell fate specification; spermatogenesis; embryonic digit morphogenesis; skeletal development; metanephros development; proximal/distal pattern formation; embryonic limb morphogenesis Disease: Radioulnar Synostosis With Amegakaryocytic Thrombocytopenia
NCBI Summary:	In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. This gene is involved in the regulation of uterine development and is required for female fertility. Mutations in this gene can cause radio-ulnar synostosis with amegakaryocytic thrombocytopenia. [provided by RefSeq, Jul 2008]
UniProt Code:	P31270
NCBI GenInfo Identifier:	13124744
NCBI Gene ID:	3207
NCBI Accession:	P31270.2
UniProt Related Accession:	P31270
Molecular Weight:
NCBI Full Name:	Homeobox protein Hox-A11
NCBI Synonym Full Names:	homeobox A11
NCBI Official Symbol:	HOXA11
NCBI Official Synonym Symbols:	HOX1; HOX1I; RUSAT1
NCBI Protein Information:	homeobox protein Hox-A11
UniProt Protein Name:	Homeobox protein Hox-A11
UniProt Synonym Protein Names:	Homeobox protein Hox-1I
UniProt Gene Name:	HOXA11
UniProt Entry Name:	HXA11_HUMAN

	Western blot analysis of extracts of various cell lines, using HOXA11 antibody (CAB2976) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of HeLa cells using HOXA11 antibody (CAB2976) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.