Antibody Name:	Anti-HOPX Antibody
Antibody SKU:	CAB15537
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-73 of human HOPX (NP_631957.1).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	mouse brain, mouse lung, rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-73 of human HOPX (NP_631957.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSAE TASG PTED QVEI LEYN FNKV DKHP DSTT LCLI AAEA GLSE EETQ KWFK QRLA KWRR SEGL PSEC RSVT D
Gene ID:	84525
Uniprot:	Q9BPY8
Cellular Location:	Nucleus
Calculated MW:	8kDa/10kDa/12kDa
Observed MW:	13kDa

Synonyms:

HOPX, CAMEO, HOD, HOP, LAGY, NECC1, OB1, SMAP31, TOTO

Background:

The protein encoded by this gene is a homeodomain protein that lacks certain conserved residues required for DNA binding. It was reported that choriocarcinoma cell lines and tissues failed to express this gene, which suggested the possible involvement of this gene in malignant conversion of placental trophoblasts. Studies in mice suggest that this protein may interact with serum response factor (SRF) and modulate SRF-dependent cardiac-specific gene expression and cardiac development. Multiple alternatively spliced transcript variants have been identified for this gene.

UniProt Protein Function:	HOPX: Atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy. May act as a tumor suppressor. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Oncoprotein; DNA-binding Chromosomal Location of Human Ortholog: 4q12 Cellular Component: nucleus Molecular Function:protein binding Biological Process: negative regulation of cell differentiation; regulation of transcription from RNA polymerase II promoter; trophectodermal cell differentiation
NCBI Summary:	The protein encoded by this gene is a homeodomain protein that lacks certain conserved residues required for DNA binding. It was reported that choriocarcinoma cell lines and tissues failed to express this gene, which suggested the possible involvement of this gene in malignant conversion of placental trophoblasts. Studies in mice suggest that this protein may interact with serum response factor (SRF) and modulate SRF-dependent cardiac-specific gene expression and cardiac development. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Feb 2009]
UniProt Code:	Q9BPY8
NCBI GenInfo Identifier:	60392394
NCBI Gene ID:	84525
NCBI Accession:	Q9BPY8.1
UniProt Secondary Accession:	Q9BPY8,Q8N0V6, Q96CI1, A8K0Z2, E9PB55, G3V294,
UniProt Related Accession:	Q9BPY8
Molecular Weight:	12,625 Da
NCBI Full Name:	Homeodomain-only protein
NCBI Synonym Full Names:	HOP homeobox
NCBI Official Symbol:	HOPX
NCBI Official Synonym Symbols:	HOD; HOP; OB1; LAGY; TOTO; CAMEO; NECC1; SMAP31
NCBI Protein Information:	homeodomain-only protein
UniProt Protein Name:	Homeodomain-only protein
UniProt Synonym Protein Names:	Lung cancer-associated Y protein; Not expressed in choriocarcinoma protein 1; Odd homeobox protein 1
Protein Family:	Homeodomain-only protein
UniProt Gene Name:	HOPX
UniProt Entry Name:	HOP_HUMAN

	Western blot analysis of extracts of various cell lines, using HOPX antibody (CAB15537) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of C6 cells using HOPX antibody (CAB15537) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using HOPX antibody (CAB15537) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using HOPX antibody (CAB15537) at dilution of 1:100. Blue: DAPI for nuclear staining.