Antibody Name:	Anti-HNRNPDL Antibody
Antibody SKU:	CAB10721
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 261-420 of human HNRNPDL (NP_112740.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, SW620, HepG2, Mouse brain, Mouse testis, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 261-420 of human HNRNPDL (NP_112740.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NIEL PMDT KTNE RRGF CFIT YTDE EPVK KLLE SRYH QIGS GKCE IKVA QPKE VYRQ QQQQ QKGG RGAA AGGR GGTR GRGR GQGQ NWNQ GFNN YYDQ GYGN YNSA YGGD QNYS GYGG YDYT GYNY GNYG YGQG YADY SGQQ STYG KASR GGGN HQNN YQPY
Gene ID:	9987
Uniprot:	O14979
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	27kDa/33kDa/46kDa
Observed MW:	38kDa

Synonyms:

HNRNPDL, HNRNP, HNRPDL, JKTBP, JKTBP2, LGMD1G, laAUF1

Background:

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two RRM domains that bind to RNAs. Three alternatively spliced transcript variants have been described for this gene. One of the variants is probably not translated because the transcript is a candidate for nonsense-mediated mRNA decay. The protein isoforms encoded by this gene are similar to its family member HNRPD.

UniProt Protein Function:	hnRNP D-like: Acts as a transcriptional regulator. Promotes transcription repression. Promotes transcription activation in differentiated myotubes. Binds to double- and single-stranded DNA sequences. Binds to the transcription suppressor CATR sequence of the COX5B promoter. Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Binds both to nuclear and cytoplasmic poly(A) mRNAs. Binds to poly(G) and poly(A), but not to poly(U) or poly(C) RNA homopolymers. Binds to the 5'-ACUAGC-3' RNA consensus sequence. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding; RNA processing; RNA-binding Chromosomal Location of Human Ortholog: 4q21.22 Cellular Component: nucleoplasm Molecular Function:protein binding; RNA binding Disease: Limb-girdle Muscular Dystrophy, Type 1g
NCBI Summary:	This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two RRM domains that bind to RNAs. Three alternatively spliced transcript variants have been described for this gene. One of the variants is probably not translated because the transcript is a candidate for nonsense-mediated mRNA decay. The protein isoforms encoded by this gene are similar to its family member HNRPD. [provided by RefSeq, May 2011]
UniProt Code:	O14979
NCBI GenInfo Identifier:	74762096
NCBI Gene ID:	9987
NCBI Accession:	O14979.3
UniProt Secondary Accession:	O14979,Q6SPF2, Q7KZ74, Q7KZ75, Q96IM0, Q96S43,
UniProt Related Accession:	O14979
Molecular Weight:	46kDa
NCBI Full Name:	Heterogeneous nuclear ribonucleoprotein D-like
NCBI Synonym Full Names:	heterogeneous nuclear ribonucleoprotein D like
NCBI Official Symbol:	HNRNPDL
NCBI Official Synonym Symbols:	HNRNP; JKTBP; HNRPDL; JKTBP2; LGMD1G; laAUF1
NCBI Protein Information:	heterogeneous nuclear ribonucleoprotein D-like
UniProt Protein Name:	Heterogeneous nuclear ribonucleoprotein D-like
UniProt Synonym Protein Names:	AU-rich element RNA-binding factor; JKT41-binding protein; Protein laAUF1
Protein Family:	Heterogeneous nuclear ribonucleoprotein
UniProt Gene Name:	HNRNPDL

	Western blot analysis of extracts of various cell lines, using HNRNPDL antibody (CAB10721) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using HNRNPDL antibody (CAB10721) at dilution of 1:100. Blue: DAPI for nuclear staining.