Antibody Name:	Anti-HNRNPA1 Antibody
Antibody SKU:	CAB7491
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-372 of human HNRNPA1 (NP_112420.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, Jurkat, HepG2, HL-60, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-372 of human HNRNPA1 (NP_112420.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSKS ESPK EPEQ LRKL FIGG LSFE TTDE SLRS HFEQ WGTL TDCV VMRD PNTK RSRG FGFV TYAT VEEV DAAM NARP HKVD GRVV EPKR AVSR EDSQ RPGA HLTV KKIF VGGI KEDT EEHH LRDY FEQY GKIE VIEI MTDR GSGK KRGF AFVT FDDH DSVD KIVI QKYH TVNG HNCE VRKA LSKQ EMAS ASSS QRGR SGSG NFGG GRGG GFGG NDNF GRGG NFSG RGGF GGSR GGGG YGGS GDGY NGFG NDGG YGGG GPGY SGGS RGYG SGGQ GYGN QGSG YGGS GSYD SYNN GGGG GFGG GSGS NFGG GGSY NDFG NYNN QSSN FGPM KGGN FGGR SSGP YGGG GQYF AKPR NQGG YGGS SSSS SYGS GRRF
Gene ID:	3178
Uniprot:	P09651
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	29kDa/34kDa/38kDa
Observed MW:	39kDa

Synonyms:

HNRNPA1, ALS19, ALS20, HNRPA1, HNRPA1L3, IBMPFD3, UP 1, hnRNP A1, hnRNP-A1

Background:

This gene encodes a member of a family of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins that associate with pre-mRNAs in the nucleus and influence pre-mRNA processing, as well as other aspects of mRNA metabolism and transport. The protein encoded by this gene is one of the most abundant core proteins of hnRNP complexes and plays a key role in the regulation of alternative splicing. Mutations in this gene have been observed in individuals with amyotrophic lateral sclerosis 20. Multiple alternatively spliced transcript variants have been found. There are numerous pseudogenes of this gene distributed throughout the genome.

UniProt Protein Function:	hnRNP A1: Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication. Identified in the spliceosome C complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with SEPT6. Interacts with HCV NS5B and with the 5'-UTR and 3'-UTR of HCV RNA. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding; RNA splicing; Spliceosome; Nuclear export Chromosomal Location of Human Ortholog: 12q13.1 Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus; ribonucleoprotein complex; spliceosome Molecular Function:protein binding; RNA binding; single-stranded DNA binding; single-stranded RNA binding Biological Process: fibroblast growth factor receptor signaling pathway; gene expression; mRNA processing; negative regulation of telomere maintenance via telomerase; nuclear export; nuclear import; nuclear mRNA splicing, via spliceosome; positive regulation of telomere maintenance via telomerase; RNA export from nucleus Disease: Amyotrophic Lateral Sclerosis 20; Inclusion Body Myopathy With Early-onset Paget Disease With Or Without Frontotemporal Dementia 3
NCBI Summary:	This gene encodes a member of a family of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins that associate with pre-mRNAs in the nucleus and influence pre-mRNA processing, as well as other aspects of mRNA metabolism and transport. The protein encoded by this gene is one of the most abundant core proteins of hnRNP complexes and plays a key role in the regulation of alternative splicing. Mutations in this gene have been observed in individuals with amyotrophic lateral sclerosis 20. Multiple alternatively spliced transcript variants have been found. There are numerous pseudogenes of this gene distributed throughout the genome. [provided by RefSeq, Feb 2016]
UniProt Code:	P09651
NCBI GenInfo Identifier:	288558857
NCBI Gene ID:	3178
NCBI Accession:	P09651.5
UniProt Secondary Accession:	P09651,Q3MIB7, Q6PJZ7, A8K4Z8,
UniProt Related Accession:	P09651
Molecular Weight:	29,386 Da
NCBI Full Name:	Heterogeneous nuclear ribonucleoprotein A1
NCBI Synonym Full Names:	heterogeneous nuclear ribonucleoprotein A1
NCBI Official Symbol:	HNRNPA1
NCBI Official Synonym Symbols:	UP 1; ALS19; ALS20; HNRPA1; IBMPFD3; HNRPA1L3; hnRNP A1; hnRNP-A1
NCBI Protein Information:	heterogeneous nuclear ribonucleoprotein A1
UniProt Protein Name:	Heterogeneous nuclear ribonucleoprotein A1
UniProt Synonym Protein Names:	Helix-destabilizing protein; Single-strand RNA-binding protein; hnRNP core protein A1
Protein Family:	Heterogeneous nuclear ribonucleoproteins
UniProt Gene Name:	HNRNPA1
UniProt Entry Name:	ROA1_HUMAN

	Western blot analysis of extracts of various cell lines, using HNRNPA1 antibody (CAB7491) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min.
	Immunohistochemistry of paraffin-embedded rat brain using HNRNPA1 Antibody (CAB7491) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using HNRNPA1 Antibody (CAB7491) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPA1 Antibody (CAB7491) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using HNRNPA1 antibody (CAB7491).