Antibody Name:	Anti-HNRNPA0 Antibody
Antibody SKU:	CAB6029
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HNRNPA0 (NP_006796.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF-7, BT-474, SKOV3, 293T, HeLa, Mouse brain, Mouse spleen, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HNRNPA0 (NP_006796.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MENS QLCK LFIG GLNV QTSE SGLR GHFE AFGT LTDC VVVV NPQT KRSR CFGF VTYS NVEE ADAA MAAS PHAV DGNT VELK RAVS REDS ARPG AHAK VKKL FVGG LKGD VAEG DLIE HFSQ FGTV EKAE IIAD KQSG KKRG FGFV YFQN HDAA DKAA VVKF HPIQ GHRV EVKK AVPK EDIY
Gene ID:	10949
Uniprot:	Q13151
Cellular Location:	Nucleus
Calculated MW:	30kDa
Observed MW:	37kDa

Synonyms:

HNRNPA0, HNRPA0

Background:

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus.

UniProt Protein Function:	hnRNP A0: a ubiquitously expressed heterogeneous nuclear ribonucleoprotein (hnRNP) of the A/B subfamily. hnRNPs are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. Has two repeats of RNA recognition motif domains (RRM), followed by a glycine-rich C-terminus.
UniProt Protein Details:	Protein type:RNA splicing; RNA-binding Chromosomal Location of Human Ortholog: 5q31 Cellular Component: nucleoplasm; nucleus; ribonucleoprotein complex Molecular Function:RNA binding; nucleotide binding; AU-rich element binding; protein kinase binding Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; response to lipopolysaccharide; inflammatory response; mRNA processing
NCBI Summary:	This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13151
NCBI GenInfo Identifier:	8134660
NCBI Gene ID:	10949
NCBI Accession:	Q13151.1
UniProt Secondary Accession:	Q13151,Q6IB18,
UniProt Related Accession:	Q13151
Molecular Weight:	30,841 Da
NCBI Full Name:	Heterogeneous nuclear ribonucleoprotein A0
NCBI Synonym Full Names:	heterogeneous nuclear ribonucleoprotein A0
NCBI Official Symbol:	HNRNPA0
NCBI Official Synonym Symbols:	HNRPA0
NCBI Protein Information:	heterogeneous nuclear ribonucleoprotein A0; hnRNA binding protein; hnRNP A0
UniProt Protein Name:	Heterogeneous nuclear ribonucleoprotein A0
Protein Family:	Heterogeneous nuclear ribonucleoprotein
UniProt Gene Name:	HNRNPA0
UniProt Entry Name:	ROA0_HUMAN

	Western blot analysis of extracts of various cell lines, using HNRNPA0 antibody (CAB6029) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human esophagus using HNRNPA0 antibody (CAB6029) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L-929 cells using HNRNPA0 Polyclonal Antibody (CAB6029) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug HNRNPA0 antibody (CAB6029). Western blot was performed from the immunoprecipitate using HNRNPA0 antibody (CAB6029) at a dilition of 1:1000.