Name: Anti-HNF4G Antibody (CAB16051)
Brand: Epigenetics & Nuclear Signaling Antibodies 2
SKU: CAB16051
Availability: OutOfStock

Antibody Name:	Anti-HNF4G Antibody
Antibody SKU:	CAB16051
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 366-445 of human HNF4G (NP_004124.4).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, Mouse pancreas, Rat pancreas, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 366-445 of human HNF4G (NP_004124.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ASND GSHL HHPM HPHL SQDP LTGQ TILL GPMS TLVH ADQI STPE TPLP SPPQ GSGQ EQYK IAAN QASV ISHQ HLSK QKQL
Gene ID:	3174
Uniprot:	Q14541
Cellular Location:	Nucleus
Calculated MW:	45kDa/50kDa
Observed MW:	50kDa

Synonyms:	HNF4G, NR2A2, NR2A3
Background:

UniProt Protein Function:	HNF4G: Transcription factor. Has a lower transcription activation potential than HNF4-alpha. Belongs to the nuclear hormone receptor family. NR2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor; DNA-binding Chromosomal Location of Human Ortholog: 8q21.11 Cellular Component: nucleoplasm Molecular Function:transcription factor activity Biological Process: regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter
UniProt Code:	Q14541
NCBI GenInfo Identifier:	160110004
NCBI Gene ID:	3174
NCBI Accession:	Q14541.3
UniProt Secondary Accession:	Q14541,Q7Z2V9, Q9UH81, Q9UIS6,
UniProt Related Accession:	Q14541
Molecular Weight:	50,041 Da
NCBI Full Name:	Hepatocyte nuclear factor 4-gamma
NCBI Synonym Full Names:	hepatocyte nuclear factor 4 gamma
NCBI Official Symbol:	HNF4G
NCBI Official Synonym Symbols:	NR2A2; NR2A3
NCBI Protein Information:	hepatocyte nuclear factor 4-gamma
UniProt Protein Name:	Hepatocyte nuclear factor 4-gamma
UniProt Synonym Protein Names:	Nuclear receptor subfamily 2 group A member 2
Protein Family:	Hepatocyte nuclear factor
UniProt Gene Name:	HNF4G
UniProt Entry Name:	HNF4G_HUMAN

	Western blot analysis of extracts of various cell lines, using HNF4G antibody (CAB16051) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunofluorescence analysis of U-2 OS cells using HNF4G Polyclonal Antibody (CAB16051) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.