Cell Biology Antibodies 3

Anti-HMOX2 Antibody (CAB12713)

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SKU:
CAB12713
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-HMOX2 Antibody
Antibody SKU:CAB12713
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human HMOX2 (NP_002125.3).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T, A-549, SKOV3, HT-29, LO2, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human HMOX2 (NP_002125.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSAE VETS EGVD ESEK KNSG ALEK ENQM RMAD LSEL LKEG TKEA HDRA ENTQ FVKD FLKG NIKK ELFK LATT ALYF TYSA LEEE MERN KDHP AFAP LYFP MELH RKEA LTKD MEYF FGEN WEEQ VQCP KAAQ KYVE RIHY IGQN EPEL LVAH AYTR YMGD LSGG QVLK KVAQ RALK LPST GEGT QFYL FENV DNAQ QFKQ LYRA RMNA LDLN MKTK ERIV EEAN KAFE YNMQ IFNE LDQA GSTL ARET LEDG FPVH DGKG DMRK CPFY AA
Gene ID:3163
Uniprot:P30519
Cellular Location:Endoplasmic reticulum, Microsome
Calculated MW:32kDa/36kDa
Observed MW:36kDa
Synonyms:HMOX2, HO-2
Background:Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene.
UniProt Protein Function:HMOX2: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter. Belongs to the heme oxygenase family.
UniProt Protein Details:

Protein type:Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.14.99.3; Endoplasmic reticulum; Oxidoreductase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: endoplasmic reticulum membrane; membrane; plasma membrane

Molecular Function:heme binding; heme oxygenase (decyclizing) activity; protein binding

Biological Process: cellular iron ion homeostasis; heme catabolic process; heme oxidation; iron ion homeostasis; response to hypoxia; response to oxidative stress

NCBI Summary:Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]
UniProt Code:P30519
NCBI GenInfo Identifier:1170328
NCBI Gene ID:3163
NCBI Accession:P30519.2
UniProt Secondary Accession:P30519,O60605, A8MT35, D3DUD5, I3L430,
UniProt Related Accession:P30519
Molecular Weight:32,837 Da
NCBI Full Name:Heme oxygenase 2
NCBI Synonym Full Names:heme oxygenase 2
NCBI Official Symbol:HMOX2  
NCBI Official Synonym Symbols:HO-2  
NCBI Protein Information:heme oxygenase 2
UniProt Protein Name:Heme oxygenase 2
UniProt Gene Name:HMOX2  
Anti-HMOX2 Antibody (CAB12713)Western blot analysis of extracts of various cell lines, using HMOX2 antibody (CAB12713) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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