KO Validated Antibodies 1

Anti-HMMR Antibody (CAB12445)[KO Validated]

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SKU:
CAB12445
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-HMMR Antibody [KO Validated]
Antibody SKU:CAB12445
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human HMMR (NP_001136028.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:SW480, HeLa, Jurkat, ES-2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human HMMR (NP_001136028.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSFP KAPL KRFN DPSG CAPS PGAY DVKT LEVL KGPV SFQK SQRF KQQK ESKQ NLNV DKDT TLPA SARK VKSS ESKK ESQK NDKD LKIL EKEI RVLL QERG AQDR RIQD LETE LEKM EARL NAAL REKT SLSA NNAT LEKQ LIEL TRTN ELLK SKFS ENGN QKNL RILS LELM KLRN KRET KMRG MMAK QEGM EMKL QVTQ RSLE ESQG KIAQ LEGK LVSI EKEK IDEK SETE KLLE YIEE ISCA SDQV EKYK LDIA QLEE NLKE KNDE ILSL KQSL EENI VILS KQVE DLNV KCQL LEKE
Gene ID:3161
Uniprot:O75330
Cellular Location:Cell surface, Cytoplasm
Calculated MW:74kDa/82kDa/84kDa
Observed MW:84kDa
Synonyms:HMMR, CD168, IHABP, RHAMM
Background:The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
UniProt Protein Function:HMMR: Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular- regulated kinase (ERK) activity. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Receptor, misc.

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function:hyaluronic acid binding

Biological Process: glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; hyaluronan metabolic process; hyaluronan catabolic process

Disease: Breast Cancer

NCBI Summary:The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Dec 2008]
UniProt Code:O75330
NCBI GenInfo Identifier:145559482
NCBI Gene ID:3161
NCBI Accession:O75330.2
UniProt Secondary Accession:O75330,Q32N02, Q92767, A8K3G2, B4E114, D3DQK9, D3DQL0 E9PCS0,
UniProt Related Accession:O75330
Molecular Weight:
NCBI Full Name:Hyaluronan mediated motility receptor
NCBI Synonym Full Names:hyaluronan-mediated motility receptor (RHAMM)
NCBI Official Symbol:HMMR  
NCBI Official Synonym Symbols:CD168; IHABP; RHAMM  
NCBI Protein Information:hyaluronan mediated motility receptor; receptor for hyaluronan-mediated motility; intracellular hyaluronic acid-binding protein
UniProt Protein Name:Hyaluronan mediated motility receptor
UniProt Synonym Protein Names:Intracellular hyaluronic acid-binding protein; Receptor for hyaluronan-mediated motility; CD_antigen: CD168
Protein Family:Hyaluronan mediated motility receptor
UniProt Gene Name:HMMR  
UniProt Entry Name:HMMR_HUMAN
Anti-HMMR Antibody [KO Validated] (CAB12445)Western blot analysis of extracts of various cell lines, using HMMR antibody (CAB12445) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-HMMR Antibody [KO Validated] (CAB12445)Western blot analysis of extracts from normal (control) and HMMR Rabbit pAb knockout (KO) HeLa cells, using HMMR Rabbit pAb antibody (CAB12445) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-HMMR Antibody [KO Validated] (CAB12445)Immunofluorescence analysis of U2OS cells using HMMR antibody (CAB12445). Blue: DAPI for nuclear staining.
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