Epigenetics & Nuclear Signaling Antibodies 1

Anti-HMGN3 Antibody (CAB12649)

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SKU:
CAB12649
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-HMGN3 Antibody
Antibody SKU:CAB12649
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-77 of human HMGN3 (NP_620058.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-77 of human HMGN3 (NP_620058.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPKR KSPE NTEG KDGS KVTK QEPT RRSA RLSA KPAP PKPE PKPR KTSA KKEP GAKI SRGA KGKK EEKQ EAGK EGTE N
Gene ID:9324
Uniprot:Q15651
Cellular Location:Nucleus
Calculated MW:8kDa/10kDa
Observed MW:12kDa
Synonyms:HMGN3, PNAS-24, PNAS-25, TRIP7
Background:The protein encoded by this gene binds thyroid hormone receptor beta in the presence of thyroid hormone. The encoded protein, a member of the HMGN protein family, is thought to reduce the compactness of the chromatin fiber in nucleosomes, thereby enhancing transcription from chromatin templates. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is a related pseudogene on chromosome 1.
UniProt Protein Function:HMGN3: Binds to nucleosomes, regulating chromatin structure and consequently, chromatin-dependent processes such as transcription, DNA replication and DNA repair. Affects both insulin and glucagon levels and modulates the expression of pancreatic genes involved in insulin secretion. Regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors. Regulates the expression of SLC6A9, a glycine transporter which regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. May play a role in ocular development and astrocyte function. Belongs to the HMGN family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6q14.1

Cellular Component: cytoplasm; nucleoplasm

NCBI Summary:The protein encoded by this gene binds thyroid hormone receptor beta in the presence of thyroid hormone. The encoded protein, a member of the HMGN protein family, is thought to reduce the compactness of the chromatin fiber in nucleosomes, thereby enhancing transcription from chromatin templates. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is a related pseudogene on chromosome 1. [provided by RefSeq, Jan 2016]
UniProt Code:Q15651
NCBI GenInfo Identifier:23831169
NCBI Gene ID:9324
NCBI Accession:Q15651.2
UniProt Secondary Accession:Q15651,Q5HYD3, Q7RTT0, Q969M5, Q9BZT7, B2RD37,
UniProt Related Accession:Q15651
Molecular Weight:8,378 Da
NCBI Full Name:High mobility group nucleosome-binding domain-containing protein 3
NCBI Synonym Full Names:high mobility group nucleosomal binding domain 3
NCBI Official Symbol:HMGN3  
NCBI Official Synonym Symbols:TRIP7; PNAS-24; PNAS-25  
NCBI Protein Information:high mobility group nucleosome-binding domain-containing protein 3
UniProt Protein Name:High mobility group nucleosome-binding domain-containing protein 3
UniProt Synonym Protein Names:Thyroid receptor-interacting protein 7; TR-interacting protein 7; TRIP-7
Protein Family:High mobility group nucleosome-binding domain-containing protein
UniProt Gene Name:HMGN3  
UniProt Entry Name:HMGN3_HUMAN
Anti-HMGN3 Antibody (CAB12649)Western blot analysis of extracts of mouse kidney, using HMGN3 antibody (CAB12649) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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