Cell Biology Antibodies 10

Anti-HMGN2 Antibody (CAB6156)

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SKU:
CAB6156
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-HMGN2 Antibody
Antibody SKU:CAB6156
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human HMGN2 (NP_005508.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human
Positive Samples:293T, HepG2, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human HMGN2 (NP_005508.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPKR KAEG DAKG DKAK VKDE PQRR SARL SAKP APPK PEPK PKKA PAKK GEKV PKGK KGKA DAGK EGNN PAEN GDAK TDQA QKAE GAGD AK
Gene ID:3151
Uniprot:P05204
Cellular Location:Cytoplasm, Nucleus
Calculated MW:9kDa
Observed MW:17kDa
Synonyms:HMGN2, HMG17
Background:The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMGN1, the encoded protein may help maintain an open chromatin configuration around transcribable genes. The protein has also been found to have antimicrobial activity against bacteria, viruses and fungi.
UniProt Protein Function:HMGN2: Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Belongs to the HMGN family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 1p36.1

Cellular Component: cytoplasm; nucleus; chromatin

Molecular Function:nucleosomal DNA binding

NCBI Summary:The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMGN1, the encoded protein may help maintain an open chromatin configuration around transcribable genes. The protein has also been found to have antimicrobial activity against bacteria, viruses and fungi. [provided by RefSeq, Oct 2014]
UniProt Code:P05204
NCBI GenInfo Identifier:123106
NCBI Gene ID:3151
NCBI Accession:P05204.3
UniProt Secondary Accession:P05204,Q0VGD5, Q6FGI5, Q96C64,
UniProt Related Accession:P05204
Molecular Weight:90
NCBI Full Name:Non-histone chromosomal protein HMG-17
NCBI Synonym Full Names:high mobility group nucleosomal binding domain 2
NCBI Official Symbol:HMGN2  
NCBI Official Synonym Symbols:HMG17  
NCBI Protein Information:non-histone chromosomal protein HMG-17; high mobility group protein N2; nonhistone chromosomal protein HMG-17; high-mobility group nucleosomal binding domain 2; high-mobility group (nonhistone chromosomal) protein 17; high mobility group nucleosome-binding domain-containing protein 2
UniProt Protein Name:Non-histone chromosomal protein HMG-17
UniProt Synonym Protein Names:High mobility group nucleosome-binding domain-containing protein 2
Protein Family:Non-histone chromosomal protein
UniProt Gene Name:HMGN2  
UniProt Entry Name:HMGN2_HUMAN
Anti-HMGN2 Antibody (CAB6156)Western blot analysis of extracts of various cell lines, using HMGN2 antibody (CAB6156) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 40s.
Anti-HMGN2 Antibody (CAB6156)Immunofluorescence analysis of HeLa cells using HMGN2 antibody (CAB6156).
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