Cell Biology Antibodies 10
Anti-HMGN1 Antibody (CAB6155)
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- SKU:
- CAB6155
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-HMGN1 Antibody |
| Antibody SKU: | CAB6155 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGN1 (NP_004956.5). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, HepG2, Mouse kidney |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGN1 (NP_004956.5). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPKR KVSS AEGA AKEE PKRR SARL SAKP PAKV EAKP KKAA AKDK SSDK KVQT KGKR GAKG KQAE VANQ ETKE DLPA ENGE TKTE ESPA SDEA GEKE AKSD |
| Gene ID: | 3150 |
| Uniprot: | P05114 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 10kDa |
| Observed MW: | 17kDa |
| Synonyms: | HMG14, HMGN1 |
| Background: | The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. |
| UniProt Protein Function: | HMGN1: a nonhistone chromosomal protein of the HMG-14/HMG-17 protein family. Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. |
| UniProt Protein Details: | Protein type:DNA-binding; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 21q22.2 Cellular Component: nucleoplasm; nucleus Molecular Function:DNA binding Biological Process: positive regulation of RNA elongation; transcription-coupled nucleotide-excision repair |
| NCBI Summary: | The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P05114 |
| NCBI GenInfo Identifier: | 123101 |
| NCBI Gene ID: | 3150 |
| NCBI Accession: | P05114.3 |
| UniProt Secondary Accession: | P05114,Q3KQR8, |
| UniProt Related Accession: | P05114 |
| Molecular Weight: | 10,659 Da |
| NCBI Full Name: | Non-histone chromosomal protein HMG-14 |
| NCBI Synonym Full Names: | high mobility group nucleosome binding domain 1 |
| NCBI Official Symbol: | HMGN1 |
| NCBI Official Synonym Symbols: | HMG14 |
| NCBI Protein Information: | non-histone chromosomal protein HMG-14 |
| UniProt Protein Name: | Non-histone chromosomal protein HMG-14 |
| UniProt Synonym Protein Names: | High mobility group nucleosome-binding domain-containing protein 1 |
| Protein Family: | Non-histone chromosomal protein |
| UniProt Gene Name: | HMGN1 |
 | Western blot analysis of extracts of various cell lines, using HMGN1 antibody (CAB6155) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human stomach using HMGN1 antibody (CAB6155) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung cancer using HMGN1 antibody (CAB6155) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using HMGN1 antibody (CAB6155) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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