Metabolism Antibodies 2

Anti-HMGCR Antibody (CAB16875)

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SKU:
CAB16875
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-HMGCR Antibody
Antibody SKU:CAB16875
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human HMGCR.
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HeLa, mouse brain, rat brain
Immunogen:Recombinant protein of human HMGCR.
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:3156
Uniprot:P04035
Cellular Location:Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:92kDa/97kDa/99kDa
Observed MW:120kDa
Synonyms:HMGCR, LDLCQ3, HMGCR
Background:HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis.
UniProt Protein Details:

Protein type:EC 1.1.1.34; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Endoplasmic reticulum; Membrane protein, integral; Motility/polarity/chemotaxis; Oxidoreductase; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q13.3-q14

Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function:protein binding; hydroxymethylglutaryl-CoA reductase (NADPH) activity; protein homodimerization activity; hydroxymethylglutaryl-CoA reductase activity; coenzyme binding

Biological Process: negative regulation of MAP kinase activity; isoprenoid biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of skeletal muscle development; cellular lipid metabolic process; cholesterol biosynthetic process; positive regulation of stress-activated MAPK cascade; coenzyme A metabolic process; myoblast differentiation; ubiquinone metabolic process; response to ethanol; negative regulation of vasodilation; visual learning; protein tetramerization; response to nutrient; aging

NCBI Summary:HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:P04035
NCBI GenInfo Identifier:123343
NCBI Gene ID:3156
NCBI Accession:P04035.1
UniProt Secondary Accession:P04035,Q8N190, B7Z3Y9,
UniProt Related Accession:P04035
Molecular Weight:888
NCBI Full Name:3-hydroxy-3-methylglutaryl-coenzyme A reductase
NCBI Synonym Full Names:3-hydroxy-3-methylglutaryl-CoA reductase
NCBI Official Symbol:HMGCR  
NCBI Official Synonym Symbols:LDLCQ3  
NCBI Protein Information:3-hydroxy-3-methylglutaryl-Coenzyme A reductase; HMG-CoA reductase; hydroxymethylglutaryl-CoA reductase; 3-hydroxy-3-methylglutaryl CoA reductase (NADPH)
UniProt Protein Name:3-hydroxy-3-methylglutaryl-coenzyme A reductase
UniProt Gene Name:HMGCR  
UniProt Entry Name:HMDH_HUMAN
Anti-HMGCR Antibody (CAB16875)Western blot analysis of extracts of various cell lines, using HMGCR antibody (CAB16875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-HMGCR Antibody (CAB16875)Immunohistochemistry of paraffin-embedded human colon using HMGCR antibody (CAB16875) at dilution of 1:200 (40x lens).
Anti-HMGCR Antibody (CAB16875)Immunofluorescence analysis of NIH/3T3 cells using HMGCR antibody (CAB16875) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-HMGCR Antibody (CAB16875)Confocal immunofluorescence analysis of NIH-3T3 cells using HMGCR Polyclonal Antibody (CAB16875) at dilution of 1:200. Blue: DAPI for nuclear staining.
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