Immunology Antibodies 1

Anti-HMGB3 Antibody (CAB15064)

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SKU:
CAB15064
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-HMGB3 Antibody
Antibody SKU:CAB15064
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB3 (NP_005333.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, MCF7, mouse kidney
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB3 (NP_005333.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FFLF CSEF RPKI KSTN PGIS IGDV AKKL GEMW NNLN DSEK QPYI TKAA KLKE KYEK DVAD YKSK GKFD GAKG PAKV ARKK VEEE DEEE EEEE EEEE EEED E
Gene ID:3149
Uniprot:O15347
Cellular Location:Chromosome, Cytoplasm, Nucleus
Calculated MW:22kDa
Observed MW:23kDa
Synonyms:HMGB3, HMG-2a, HMG-4, HMG2A, HMG4
Background:This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:HMGB3: Binds preferentially single-stranded DNA and unwinds double stranded DNA. Belongs to the HMGB family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: Xq28

Molecular Function:DNA bending activity; double-stranded DNA binding; four-way junction DNA binding; protein binding

Biological Process: DNA geometric change; DNA recombination; multicellular organismal development

Disease: Microphthalmia, Syndromic 13

NCBI Summary:This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Code:O15347
NCBI GenInfo Identifier:85701353
NCBI Gene ID:3149
NCBI Accession:O15347.4
UniProt Secondary Accession:O15347,O95556, Q6NS40,
UniProt Related Accession:O15347
Molecular Weight:22,980 Da
NCBI Full Name:High mobility group protein B3
NCBI Synonym Full Names:high mobility group box 3
NCBI Official Symbol:HMGB3  
NCBI Official Synonym Symbols:HMG4; HMG-4; HMG2A; HMG-2a  
NCBI Protein Information:high mobility group protein B3
UniProt Protein Name:High mobility group protein B3
UniProt Synonym Protein Names:High mobility group protein 2a; HMG-2a; High mobility group protein 4; HMG-4
Protein Family:High mobility group protein
UniProt Gene Name:HMGB3  
Anti-HMGB3 Antibody (CAB15064)Western blot analysis of extracts of various cell lines, using HMGB3 antibody (CAB15064) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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system_update_altDatasheetHuman HMGB3 Recombinant Protein High Mobility Group Protein B3 (HMGB3) belongs to the HMGB family. Members of the HMG box subfamily are thought to be have an important r