Cell Biology Antibodies 17
Anti-HMGB2 Antibody (CAB9168)
- SKU:
- CAB9168
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-HMGB2 Antibody |
| Antibody SKU: | CAB9168 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human HMGB2 |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, RAW264.7, THP-1, Mouse testis, Rat testis, Rat spleen, Rat thymus |
| Immunogen: | A synthesized peptide derived from human HMGB2 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 3148 |
| Uniprot: | P26583 |
| Cellular Location: | |
| Calculated MW: | 24kDa |
| Observed MW: | 24KDa |
| Synonyms: | HMG2 |
| Background: | This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq, Jul 2008] |
| UniProt Protein Function: | HMGB2: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Belongs to the HMGB family. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; DNA-binding Chromosomal Location of Human Ortholog: 4q31 Cellular Component: nucleoplasm; extracellular space; protein complex; perinuclear region of cytoplasm; cytoplasm; condensed chromosome; nucleus Molecular Function:protein domain specific binding; protein binding; RAGE receptor binding; DNA binding; double-stranded DNA binding; damaged DNA binding; chromatin binding; transcription factor activity; DNA bending activity; single-stranded DNA binding; chemoattractant activity Biological Process: positive regulation of nuclease activity; establishment and/or maintenance of chromatin architecture; DNA topological change; V(D)J recombination; apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of erythrocyte differentiation; male gonad development; spermatid nuclear differentiation; base-excision repair, DNA ligation; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; nucleosome assembly; positive chemotaxis; response to steroid hormone stimulus; DNA ligation during DNA repair; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of megakaryocyte differentiation; DNA fragmentation during apoptosis; negative regulation of transcription, DNA-dependent; positive regulation of DNA binding; cell structure disassembly during apoptosis |
| NCBI Summary: | This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P26583 |
| NCBI GenInfo Identifier: | 123374 |
| NCBI Gene ID: | 3148 |
| NCBI Accession: | P26583.2 |
| UniProt Secondary Accession: | P26583,Q5U072, B2R4K8, D3DP37, |
| UniProt Related Accession: | P26583 |
| Molecular Weight: | 209 |
| NCBI Full Name: | High mobility group protein B2 |
| NCBI Synonym Full Names: | high mobility group box 2 |
| NCBI Official Symbol: | HMGB2 |
| NCBI Official Synonym Symbols: | HMG2 |
| NCBI Protein Information: | high mobility group protein B2; HMG-2; high-mobility group box 2; high mobility group protein 2; high-mobility group (nonhistone chromosomal) protein 2 |
| UniProt Protein Name: | High mobility group protein B2 |
| UniProt Synonym Protein Names: | High mobility group protein 2; HMG-2 |
| Protein Family: | High mobility group protein |
| UniProt Gene Name: | HMGB2 |
| UniProt Entry Name: | HMGB2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HMGB2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded rat testis using HMGB2 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human colon using HMGB2 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded mouse testis using HMGB2 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with IHC staining protocol. |
 | Immunofluorescence analysis of C6 cells using HMGB2 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using HMGB2 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using HMGB2 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300ug extracts of Mouse testis cells using 3ug HMGB2 antibody at a dilition of 1:1000. |
 | Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using HMGB2 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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