Antibody Name:	Anti-HMGB1 Antibody [KO Validated]
Antibody SKU:	CAB2553
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (NP_002119.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, MCF7

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (NP_002119.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SAFF LFCS EYRP KIKG EHPG LSIG DVAK KLGE MWNN TAAD DKQP YEKK AAKL KEKY EKDI AAYR AKGK PDAA KKGV VKAE KSKK KKEE EEDE EDEE DEEE E
Gene ID:	3146
Uniprot:	P09429
Cellular Location:	Cell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Endosome, Extracellular side, Nucleus, Peripheral membrane protein, Secreted
Calculated MW:	24kDa
Observed MW:	25KDa

Synonyms:

HMG-1, HMG1, HMG3, SBP-1, HMGB1

Background:

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.

UniProt Protein Function:	Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury (PubMed:27362237). Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed:23519706, PubMed:23446148, PubMed:23994764, PubMed:25048472). Has proangiogdenic activity. May be involved in platelet activation. Binds to phosphatidylserine and phosphatidylethanolamide. Bound to RAGE mediates signaling for neuronal outgrowth. May play a role in accumulation of expanded polyglutamine (polyQ) proteins such as huntingtin (HTT) or TBP (PubMed:23303669, PubMed:25549101).
NCBI Summary:	This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
UniProt Code:	P09429
NCBI GenInfo Identifier:	123369
NCBI Gene ID:	3146
NCBI Accession:	P09429.3
UniProt Secondary Accession:	P09429,Q14321, Q5T7C3, Q6IBE1, A5D8W9,
UniProt Related Accession:	P09429
Molecular Weight:	24,894 Da
NCBI Full Name:	High mobility group protein B1
NCBI Synonym Full Names:	high mobility group box 1
NCBI Official Symbol:	HMGB1
NCBI Official Synonym Symbols:	HMG1; HMG3; HMG-1; SBP-1
NCBI Protein Information:	high mobility group protein B1
UniProt Protein Name:	High mobility group protein B1
UniProt Synonym Protein Names:	High mobility group protein 1; HMG-1
Protein Family:	High mobility group protein
UniProt Gene Name:	HMGB1

	Western blot analysis of extracts of MCF7 cells, using [KO Validated] HMGB1 Rabbit pAb (CAB2553) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts from normal (control) and [KO Validated] HMGB1 Rabbit pAb knockout (KO) HeLa cells, using [KO Validated] HMGB1 Rabbit pAb antibody (CAB2553) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat brain using HMGB1 Antibody (CAB2553) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using HMGB1 Antibody (CAB2553) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using HMGB1 Antibody (CAB2553) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-HMGB1 Antibody (CAB0719)[KO Validated]	Human HMGB1 ELISA Kit
Anti-Acetyl-HMGB1-K28 Antibody (CAB16001)	Human HMGB-1 (High mobility group protein B1) CLIA Kit (HUES00881)
Anti-Acetyl-HMGB1-K29 Antibody (CAB16002)[KO Validated]	Human High mobility group protein B1 (HMGB1) ELISA Kit
Anti-Acetyl-HMGB1-K30 Antibody (CAB16003)
Anti-Acetyl-HMGB1-K90 Antibody (CAB16004)