KO Validated Antibodies 2

Anti-HMG20B Antibody (CAB19951)[KO Validated]

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SKU:
CAB19951
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-HMG20B Antibody [KO Validated]
Antibody SKU:CAB19951
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human HMG20B.
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant protein of human HMG20B.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:10362
Uniprot:Q9P0W2
Cellular Location:Chromosome, Nucleus
Calculated MW:24kDa/32kDa/35kDa
Observed MW:36KDa
Anti-HMG20B Antibody [KO Validated] (CAB19951)Western blot analysis of extracts from normal (control) and HMG20B knockout (KO) 293T cells, using HMG20B antibody (CAB19951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:HMG20B, BRAF25, BRAF35, HMGX2, HMGXB2, PP7706, SMARCE1r, SOXL, pp8857
Background:
UniProt Protein Function:HMG20B: Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; chromosome

Molecular Function:protein binding; DNA binding; protein heterodimerization activity; chromatin binding

Biological Process: chromatin remodeling; establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; blood coagulation; positive regulation of neuron differentiation; cell cycle; negative regulation of protein sumoylation

UniProt Code:Q9P0W2
NCBI GenInfo Identifier:57012878
NCBI Gene ID:10362
NCBI Accession:Q9P0W2.1
UniProt Secondary Accession:Q9P0W2,Q6IBP8, Q8NBD5, Q9HD21, Q9Y491, Q9Y4A2, A6NMS5 D6W616,
UniProt Related Accession:Q9P0W2
Molecular Weight:317
NCBI Full Name:SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related
NCBI Synonym Full Names:high mobility group 20B
NCBI Official Symbol:HMG20B  
NCBI Official Synonym Symbols:SOXL; HMGX2; BRAF25; BRAF35; HMGXB2; PP7706; pp8857; SMARCE1r  
NCBI Protein Information:SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related; SMARCE1-related protein; high-mobility group 20B; BRCA2-associated factor 35; HMG box domain containing 2; HMG box-containing protein 20B; HMG domain-containing protein 2; Sox-like transcriptional factor; HMG domain-containing protein HMGX2; structural DNA-binding protein BRAF35; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E, member 1-related
UniProt Protein Name:SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related
UniProt Synonym Protein Names:BRCA2-associated factor 35; HMG box-containing protein 20B; HMG domain-containing protein 2; HMG domain-containing protein HMGX2; Sox-like transcriptional factor; Structural DNA-binding protein BRAF35
Protein Family:SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily
UniProt Gene Name:HMG20B  
UniProt Entry Name:HM20B_HUMAN
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