Epigenetics & Nuclear Signaling Antibodies 4

Anti-HLTF Antibody (CAB8353)

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SKU:
CAB8353
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-HLTF Antibody
Antibody SKU:CAB8353
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 726-1009 of human HLTF (NP_620636.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-251MG, 293T, LO2, A-549, HeLa, HT-1080
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 726-1009 of human HLTF (NP_620636.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VSSN GPSG NDTP EELR KKLI RKMK LILS SGSD EECA ICLD SLTV PVIT HCAH VFCK PCIC QVIQ NEQP HAKC PLCR NDIH EDNL LECP PEEL ARDS EKKS DMEW TSSS KINA LMHA LTDL RKKN PNIK SLVV SQFT TFLS LIEI PLKA SGFV FTRL DGSM AQKK RVES IQCF QNTE AGSP TIML LSLK AGGV GLNL SAAS RVFL MDPA WNPA AEDQ CFDR CHRL GQKQ EVII TKFI VKDS VEEN MLKI QNKK RELA AGAF GTKK PNAD EMKQ AKIN EIRT LIDL
Gene ID:6596
Uniprot:Q14527
Cellular Location:Cytoplasm, Nucleus, nucleolus, nucleoplasm
Calculated MW:99kDa/113kDa
Observed MW:114kDa
Synonyms:HLTF, HIP116, HIP116A, HLTF1, RNF80, SMARCA3, SNF2L3, ZBU1
Background:This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein.
UniProt Protein Function:SMARCA3: an enzyme with apparent ATP-dependent DNA helicase activity. Belongs to the SNF2/RAD54 helicase family, RAD16 subfamily. Two alternatively spliced human isoforms have been described.
UniProt Protein Details:

Protein type:Ligase; EC 3.6.1.-; Nucleolus; EC 3.6.4.-; EC 6.3.2.-; Ubiquitin conjugating system; Helicase; EC 6.3.2.19; DNA-binding; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 3q25.1-q26.1

Cellular Component: membrane; nucleoplasm; nucleus

Molecular Function:DNA binding; protein binding; ubiquitin protein ligase binding

Biological Process: regulation of transcription, DNA-dependent

NCBI Summary:This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq, Jul 2008]
UniProt Code:Q14527
NCBI GenInfo Identifier:60390864
NCBI Gene ID:6596
NCBI Accession:Q14527.2
UniProt Secondary Accession:Q14527,Q14536, Q16051, Q7KYJ6, Q86YA5, Q92652, Q96KM9 D3DNH3,
UniProt Related Accession:Q14527
Molecular Weight:99,919 Da
NCBI Full Name:Helicase-like transcription factor
NCBI Synonym Full Names:helicase like transcription factor
NCBI Official Symbol:HLTF  
NCBI Official Synonym Symbols:ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3  
NCBI Protein Information:helicase-like transcription factor
UniProt Protein Name:Helicase-like transcription factor
UniProt Synonym Protein Names:DNA-binding protein/plasminogen activator inhibitor 1 regulator; HIP116; RING finger protein 80; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; Sucrose nonfermenting protein 2-like 3
Protein Family:Helicase-like transcription factor
UniProt Gene Name:HLTF  
UniProt Entry Name:HLTF_HUMAN
Anti-HLTF Polyclonal Antibody (CAB8353)Western blot analysis of extracts of various cell lines, using HLTF antibody (CAB8353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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