Immunology Antibodies 1
Anti-HLA-F Antibody (CAB10384)
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- SKU:
- CAB10384
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-HLA-F Antibody |
| Antibody SKU: | CAB10384 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-190 of human HLA-F (NP_001091949.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-190 of human HLA-F (NP_001091949.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GSHS LRYF STAV SRPG RGEP RYIA VEYV DDTQ FLRF DSDA AIPR MEPR EPWV EQEG PQYW EWTT GYAK ANAQ TDRV ALRN LLRR YNQS EAGS HTLQ GMNG CDMG PDGR LLRG YHQH AYDG KDYI SLNE DLRS WTAA DTVA QITQ RFYE AEEY AEEF RTYL EGEC LELL R |
| Gene ID: | 3134 |
| Uniprot: | P30511 |
| Cellular Location: | Membrane, Single-pass type I membrane protein |
| Calculated MW: | 28kDa/39kDa/50kDa |
| Observed MW: | 50kDa |
| Synonyms: | HLA-F, CDA12, HLA-5.4, HLA-CDA12, HLAF |
| Background: | This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain. |
| UniProt Protein Function: | HLA-F: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: Golgi membrane; phagocytic vesicle membrane; cell surface; membrane; endoplasmic reticulum; early endosome membrane; plasma membrane; MHC class I protein complex Molecular Function:TAP1 binding; peptide antigen binding; TAP2 binding; receptor binding Biological Process: regulation of immune response; antigen processing and presentation of peptide antigen via MHC class I; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I |
| NCBI Summary: | This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P30511 |
| NCBI GenInfo Identifier: | 149158700 |
| NCBI Gene ID: | 3134 |
| NCBI Accession: | NP_001091948.1 |
| UniProt Secondary Accession: | P30511,Q5JQI8, Q5JQJ1, Q5SPT5, Q860R0, Q8MGQ1, Q8WLP5 Q95HC0, Q9TP68, |
| UniProt Related Accession: | P30511 |
| Molecular Weight: | 50,438 Da |
| NCBI Full Name: | HLA class I histocompatibility antigen, alpha chain F isoform 3 |
| NCBI Synonym Full Names: | major histocompatibility complex, class I, F |
| NCBI Official Symbol: | HLA-F |
| NCBI Official Synonym Symbols: | HLAF; CDA12; HLA-5.4; HLA-CDA12 |
| NCBI Protein Information: | HLA class I histocompatibility antigen, alpha chain F |
| UniProt Protein Name: | HLA class I histocompatibility antigen, alpha chain F |
| UniProt Synonym Protein Names: | CDA12; HLA F antigen; Leukocyte antigen F; MHC class I antigen F |
| Protein Family: | HLA class I histocompatibility antigen |
| UniProt Gene Name: | HLA-F |
| UniProt Entry Name: | HLAF_HUMAN |
 | Western blot analysis of extracts of A-549 cells, using HLA-F antibody (CAB10384) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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