Immunology Antibodies 3
Anti-HLA-DQA2 Antibody (CAB9761)
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- SKU:
- CAB9761
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-HLA-DQA2 Antibody |
| Antibody SKU: | CAB9761 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-125 of human HLA-DQA2 (NP_064440.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse liver, Mouse kidney, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-125 of human HLA-DQA2 (NP_064440.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EDIV ADHV ASYG VNFY QSHG PSGQ YTHE FDGD EEFY VDLE TKET VWQL PMFS KFIS FDPQ SALR NMAV GKHT LEFM MRQS NSTA ATNE VPEV TVFS KFPV TL |
| Gene ID: | 3118 |
| Uniprot: | P01906 |
| Cellular Location: | Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane |
| Calculated MW: | 28kDa |
| Observed MW: | 28kDa |
| Synonyms: | HLA-DQA2, DX-ALPHA, HLA-DXA, HLADQA2, class II |
| Background: | This gene belongs to the HLA class II alpha chain family. The encoded protein forms a heterodimer with a class II beta chain. It is located in intracellular vesicles and plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells, macrophages) and are used to present antigenic peptides on the cell surface to be recognized by CD4 T-cells. |
| UniProt Protein Function: | HLA-DQA2: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: Golgi membrane; lysosomal membrane; integral to plasma membrane; plasma membrane; endosome membrane; trans-Golgi network membrane; MHC class II protein complex Molecular Function:MHC class II receptor activity Biological Process: cytokine and chemokine mediated signaling pathway; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; T cell receptor signaling pathway |
| NCBI Summary: | This gene belongs to the HLA class II alpha chain family. The encoded protein forms a heterodimer with a class II beta chain. It is located in intracellular vesicles and plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells, macrophages) and are used to present antigenic peptides on the cell surface to be recognized by CD4 T-cells. [provided by RefSeq, Jun 2010] |
| UniProt Code: | P01906 |
| NCBI GenInfo Identifier: | 122192 |
| NCBI Gene ID: | 3118 |
| NCBI Accession: | P01906.2 |
| UniProt Secondary Accession: | P01906,O19789, Q5SQ94, Q5SR04, A2BF37, B0V0E7, |
| UniProt Related Accession: | P01906 |
| Molecular Weight: | 28,033 Da |
| NCBI Full Name: | HLA class II histocompatibility antigen, DQ alpha 2 chain |
| NCBI Synonym Full Names: | major histocompatibility complex, class II, DQ alpha 2 |
| NCBI Official Symbol: | HLA-DQA2 |
| NCBI Official Synonym Symbols: | HLA-DXA; DX-ALPHA |
| NCBI Protein Information: | HLA class II histocompatibility antigen, DQ alpha 2 chain; DX alpha chain; MHC class II DQA2; HLA class II histocompatibility antigen, DQ(6) alpha chain |
| UniProt Protein Name: | HLA class II histocompatibility antigen, DQ alpha 2 chain |
| UniProt Synonym Protein Names: | DX alpha chain; HLA class II histocompatibility antigen, DQ(6) alpha chain; HLA-DQA1; MHC class II DQA2 |
| Protein Family: | HLA class II histocompatibility antigen |
| UniProt Gene Name: | HLA-DQA2 |
| UniProt Entry Name: | DQA2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HLA-DQA2 antibody (CAB9761) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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