Immunology Antibodies 2
Anti-HLA-DPA1 Antibody (CAB1754)
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- SKU:
- CAB1754
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-HLA-DPA1 Antibody |
| Antibody SKU: | CAB1754 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-220 of human HLA-DPA1 (NP_291032.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | Raji |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-220 of human HLA-DPA1 (NP_291032.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YAAF VQTH RPTG EFMF EFDE DEMF YVDL DKKE TVWH LEEF GQAF SFEA QGGL ANIA ILNN NLNT LIQR SNHT QATN DPPE VTVF PKEP VELG QPNT LICH IDKF FPPV LNVT WLCN GELV TEGV AESL FLPR TDYS FHKF HYLT FVPS AEDF YDCR VEHW GLDQ PLLK HWEA QEPI QMPE T |
| Gene ID: | 3113 |
| Uniprot: | P20036 |
| Cellular Location: | Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane |
| Calculated MW: | 29kDa |
| Observed MW: | 36kDa |
| Synonyms: | HLA-DPA1, DP(W3), DP(W4), HLA-DP1A, HLADP, HLASB, PLT1, class II |
| Background: | HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. |
| UniProt Protein Function: | HLA-DPA1: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: Golgi membrane; cell surface; integral to plasma membrane; lysosomal membrane; plasma membrane; trans-Golgi network membrane; endosome membrane; MHC class II protein complex Molecular Function:MHC class II receptor activity; peptide antigen binding Biological Process: positive regulation of interferon-gamma production; cytokine and chemokine mediated signaling pathway; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; positive regulation of T cell proliferation; immune response; positive regulation of T cell activation; T cell receptor signaling pathway |
| NCBI Summary: | HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P20036 |
| NCBI GenInfo Identifier: | 122204 |
| NCBI Gene ID: | 3113 |
| NCBI Accession: | P20036.1 |
| UniProt Secondary Accession: | P20036,O19722, O46883, P01905, P79554, Q2Q060, Q2Q061 Q5EY03, Q5STP1, Q6DQK4, A9YWH7, B9UKH4, |
| UniProt Related Accession: | P20036 |
| Molecular Weight: | 260 |
| NCBI Full Name: | HLA class II histocompatibility antigen, DP alpha 1 chain |
| NCBI Synonym Full Names: | major histocompatibility complex, class II, DP alpha 1 |
| NCBI Official Symbol: | HLA-DPA1 |
| NCBI Official Synonym Symbols: | PLT1; HLADP; HLASB; DP(W3); DP(W4); HLA-DP1A |
| NCBI Protein Information: | HLA class II histocompatibility antigen, DP alpha 1 chain; HLA-SB alpha chain; MHC class II antigen; MHC class II DP3-alpha; Primed lymphocyte test-1; MHC class II HLA-DPA1 antigen |
| UniProt Protein Name: | HLA class II histocompatibility antigen, DP alpha 1 chain |
| UniProt Synonym Protein Names: | DP(W3); DP(W4); HLA-SB alpha chain; MHC class II DP3-alpha; MHC class II DPA1 |
| Protein Family: | HLA class II histocompatibility antigen |
| UniProt Gene Name: | HLA-DPA1 |
| UniProt Entry Name: | DPA1_HUMAN |
 | Western blot analysis of extracts of Raji cells, using HLA-DPA1 antibody (CAB1754) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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