Immunology Antibodies 1
Anti-HLA-DOB Antibody (CAB12263)
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- SKU:
- CAB12263
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-HLA-DOB Antibody |
| Antibody SKU: | CAB12263 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 27-224 of human HLA-DOB (NP_002111.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, Mouse kidney, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 27-224 of human HLA-DOB (NP_002111.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TDSP EDFV IQAK ADCY FTNG TEKV QFVV RFIF NLEE YVRF DSDV GMFV ALTK LGQP DAEQ WNSR LDLL ERSR QAVD GVCR HNYR LGAP FTVG RKVQ PEVT VYPE RTPL LHQH NLLH CSVT GFYP GDIK IKWF LNGQ EERA GVMS TGPI RNGD WTFQ TVVM LEMT PELG HVYT CLVD HSSL LSPV SVEW RAQS EYSW RK |
| Gene ID: | 3112 |
| Uniprot: | P13765 |
| Cellular Location: | Endosome membrane, Lysosome membrane, Single-pass type I membrane protein |
| Calculated MW: | 30kDa |
| Observed MW: | 36kDa |
| Synonyms: | HLA-DOB, DOB, HLA_DOB, major histocompatibility complex, class II, DO beta |
| Background: | HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. |
| UniProt Protein Function: | HLA-DOB: Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM. Belongs to the MHC class II family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.32 Cellular Component: lysosomal membrane; lysosome; MHC class II protein complex Molecular Function:MHC class II receptor activity Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; negative regulation of antigen processing and presentation of peptide antigen via MHC class II |
| NCBI Summary: | HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P13765 |
| NCBI GenInfo Identifier: | 122261 |
| NCBI Gene ID: | 3112 |
| NCBI Accession: | P13765.1 |
| UniProt Secondary Accession: | P13765,Q29746, Q29825, Q6FHC2, B0V0Y0, |
| UniProt Related Accession: | P13765 |
| Molecular Weight: | 31kDa |
| NCBI Full Name: | HLA class II histocompatibility antigen, DO beta chain |
| NCBI Synonym Full Names: | major histocompatibility complex, class II, DO beta |
| NCBI Official Symbol: | HLA-DOB |
| NCBI Official Synonym Symbols: | DOB; HLA_DOB |
| NCBI Protein Information: | HLA class II histocompatibility antigen, DO beta chain |
| UniProt Protein Name: | HLA class II histocompatibility antigen, DO beta chain |
| UniProt Synonym Protein Names: | MHC class II antigen DOB |
| Protein Family: | HLA class II histocompatibility antigen |
| UniProt Gene Name: | HLA-DOB |
 | Western blot analysis of extracts of various cell lines, using HLA-DOB antibody (CAB12263) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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