Immunology Antibodies 2
Anti-HLA-DOA Antibody (CAB6923)
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- SKU:
- CAB6923
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-HLA-DOA Antibody |
| Antibody SKU: | CAB6923 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-135 of human HLA-DOA (NP_002110.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | BT-474, Raji, MCF7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-135 of human HLA-DOA (NP_002110.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TKAD HMGS YGPA FYQS YGAS GQFT HEFD EEQL FSVD LKKS EAVW RLPE FGDF ARFD PQGG LAGI AAIK AHLD ILVE RSNR SRAI NVPP RVTV LPKS RVEL GQPN ILIC IV |
| Gene ID: | 3111 |
| Uniprot: | P06340 |
| Cellular Location: | Endosome membrane, Lysosome membrane, Single-pass type I membrane protein |
| Calculated MW: | 27kDa |
| Observed MW: | 36kDa |
| Synonyms: | HLA-DOA, HLA-DNA, HLA-DZA, HLADZ, major histocompatibility complex, class II, DO alpha |
| Background: | HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. |
| UniProt Protein Function: | HLA-DOA: Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM. Belongs to the MHC class II family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: endosome membrane; integral to membrane; lysosomal membrane; MHC class II protein complex; plasma membrane Molecular Function:MHC class II receptor activity; protein binding Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; negative regulation of antigen processing and presentation of peptide antigen via MHC class II; regulation of T cell differentiation; signal transduction |
| NCBI Summary: | HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P06340 |
| NCBI GenInfo Identifier: | 122210 |
| NCBI Gene ID: | 3111 |
| NCBI Accession: | P06340.1 |
| UniProt Secondary Accession: | P06340,Q58HU0, Q58HU1, Q5STC7, Q9TQC6, Q9TQC7, Q9TQC8 Q9TQC9, Q9TQD0, Q9TQD1, Q9TQD2, Q9TQD3, |
| UniProt Related Accession: | P06340 |
| Molecular Weight: | 27,599 Da |
| NCBI Full Name: | HLA class II histocompatibility antigen, DO alpha chain |
| NCBI Synonym Full Names: | major histocompatibility complex, class II, DO alpha |
| NCBI Official Symbol: | HLA-DOA |
| NCBI Official Synonym Symbols: | HLADZ; HLA-DNA; HLA-DZA |
| NCBI Protein Information: | HLA class II histocompatibility antigen, DO alpha chain |
| UniProt Protein Name: | HLA class II histocompatibility antigen, DO alpha chain |
| UniProt Synonym Protein Names: | MHC DN-alpha; MHC DZ alpha; MHC class II antigen DOA |
| Protein Family: | HLA class II histocompatibility antigen |
| UniProt Gene Name: | HLA-DOA |
| UniProt Entry Name: | DOA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HLA-DOA antibody (CAB6923) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5s. |
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