Name: Anti-HLA-DOA Antibody (CAB6923)
Brand: Immunology Antibodies 2
SKU: CAB6923
Availability: OutOfStock

Antibody Name:	Anti-HLA-DOA Antibody
Antibody SKU:	CAB6923
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 26-135 of human HLA-DOA (NP_002110.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	BT-474, Raji, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 26-135 of human HLA-DOA (NP_002110.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TKAD HMGS YGPA FYQS YGAS GQFT HEFD EEQL FSVD LKKS EAVW RLPE FGDF ARFD PQGG LAGI AAIK AHLD ILVE RSNR SRAI NVPP RVTV LPKS RVEL GQPN ILIC IV
Gene ID:	3111
Uniprot:	P06340
Cellular Location:	Endosome membrane, Lysosome membrane, Single-pass type I membrane protein
Calculated MW:	27kDa
Observed MW:	36kDa

Synonyms:	HLA-DOA, HLA-DNA, HLA-DZA, HLADZ, major histocompatibility complex, class II, DO alpha
Background:	HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level.

UniProt Protein Function:	HLA-DOA: Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM. Belongs to the MHC class II family.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: endosome membrane; integral to membrane; lysosomal membrane; MHC class II protein complex; plasma membrane Molecular Function:MHC class II receptor activity; protein binding Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; negative regulation of antigen processing and presentation of peptide antigen via MHC class II; regulation of T cell differentiation; signal transduction
NCBI Summary:	HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. [provided by RefSeq, Jul 2008]
UniProt Code:	P06340
NCBI GenInfo Identifier:	122210
NCBI Gene ID:	3111
NCBI Accession:	P06340.1
UniProt Secondary Accession:	P06340,Q58HU0, Q58HU1, Q5STC7, Q9TQC6, Q9TQC7, Q9TQC8 Q9TQC9, Q9TQD0, Q9TQD1, Q9TQD2, Q9TQD3,
UniProt Related Accession:	P06340
Molecular Weight:	27,599 Da
NCBI Full Name:	HLA class II histocompatibility antigen, DO alpha chain
NCBI Synonym Full Names:	major histocompatibility complex, class II, DO alpha
NCBI Official Symbol:	HLA-DOA
NCBI Official Synonym Symbols:	HLADZ; HLA-DNA; HLA-DZA
NCBI Protein Information:	HLA class II histocompatibility antigen, DO alpha chain
UniProt Protein Name:	HLA class II histocompatibility antigen, DO alpha chain
UniProt Synonym Protein Names:	MHC DN-alpha; MHC DZ alpha; MHC class II antigen DOA
Protein Family:	HLA class II histocompatibility antigen
UniProt Gene Name:	HLA-DOA
UniProt Entry Name:	DOA_HUMAN

Western blot analysis of extracts of various cell lines, using HLA-DOA antibody (CAB6923) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5s.

Antibodies

Anti-HLA-DOA Antibody (CAB15277)

HLA-DOA Antibody (PACO00915)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)