Immunology Antibodies 1
Anti-HLA-DOA Antibody (CAB15277)
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- SKU:
- CAB15277
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-HLA-DOA Antibody |
| Antibody SKU: | CAB15277 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-217 of human HLA-DOA (NP_002110.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse lung, Mouse thymus, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-217 of human HLA-DOA (NP_002110.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TKAD HMGS YGPA FYQS YGAS GQFT HEFD EEQL FSVD LKKS EAVW RLPE FGDF ARFD PQGG LAGI AAIK AHLD ILVE RSNR SRAI NVPP RVTV LPKS RVEL GQPN ILIC IVDN IFPP VINI TWLR NGQT VTEG VAQT SFYS QPDH LFRK FHYL PFVP SAED VYDC QVEH WGLD APLL RHWE LQVP IPPP DAME |
| Gene ID: | 3111 |
| Uniprot: | P06340 |
| Cellular Location: | Endosome membrane, Lysosome membrane, Single-pass type I membrane protein |
| Calculated MW: | 27kDa |
| Observed MW: | 28kDa |
| Synonyms: | HLA-DOA, HLA-DNA, HLA-DZA, HLADZ, major histocompatibility complex, class II, DO alpha |
| Background: | HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. |
| UniProt Protein Function: | HLA-DOA: Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM. Belongs to the MHC class II family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: endosome membrane; integral to membrane; lysosomal membrane; MHC class II protein complex; plasma membrane Molecular Function:MHC class II receptor activity; protein binding Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; negative regulation of antigen processing and presentation of peptide antigen via MHC class II; regulation of T cell differentiation; signal transduction |
| NCBI Summary: | HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P06340 |
| NCBI GenInfo Identifier: | 122210 |
| NCBI Gene ID: | 3111 |
| NCBI Accession: | P06340.1 |
| UniProt Secondary Accession: | P06340,Q58HU0, Q58HU1, Q5STC7, Q9TQC6, Q9TQC7, Q9TQC8 Q9TQC9, Q9TQD0, Q9TQD1, Q9TQD2, Q9TQD3, |
| UniProt Related Accession: | P06340 |
| Molecular Weight: | 27,599 Da |
| NCBI Full Name: | HLA class II histocompatibility antigen, DO alpha chain |
| NCBI Synonym Full Names: | major histocompatibility complex, class II, DO alpha |
| NCBI Official Symbol: | HLA-DOA |
| NCBI Official Synonym Symbols: | HLADZ; HLA-DNA; HLA-DZA |
| NCBI Protein Information: | HLA class II histocompatibility antigen, DO alpha chain |
| UniProt Protein Name: | HLA class II histocompatibility antigen, DO alpha chain |
| UniProt Synonym Protein Names: | MHC DN-alpha; MHC DZ alpha; MHC class II antigen DOA |
| Protein Family: | HLA class II histocompatibility antigen |
| UniProt Gene Name: | HLA-DOA |
| UniProt Entry Name: | DOA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HLA-DOA antibody (CAB15277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90S. |
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