Antibody Name:	Anti-HLA-DMB Antibody
Antibody SKU:	CAB8384
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-218 of human HLA-DMB (NP_002109.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human
Positive Samples:	Raji

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-218 of human HLA-DMB (NP_002109.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GGFV AHVE STCL LDDA GTPK DFTY CISF NKDL LTCW DPEE NKMA PCEF GVLN SLAN VLSQ HLNQ KDTL MQRL RNGL QNCA THTQ PFWG SLTN RTRP PSVQ VAKT TPFN TREP VMLA CYVW GFYP AEVT ITWR KNGK LVMP HSSA HKTA QPNG DWTY QTLS HLAL TPSY GDTY TCVV EHTG APEP ILRD WTPG LSPM QTLK
Gene ID:	3109
Uniprot:	P28068
Cellular Location:	Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein
Calculated MW:	28kDa
Observed MW:	29kDa

Synonyms:

HLA-DMB, D6S221E, RING7, major histocompatibility complex, class II, DM beta

Background:

HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.

UniProt Protein Function:	HLA-DMB: Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Belongs to the MHC class II family.
UniProt Protein Details:	Protein type:Vesicle; Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: lysosomal membrane; late endosome membrane; integral to membrane; MHC class II protein complex Biological Process: peptide antigen assembly with MHC class II protein complex; antigen processing and presentation of exogenous peptide antigen via MHC class II; positive regulation of T cell proliferation; immune response; MHC class II protein complex assembly
NCBI Summary:	HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
UniProt Code:	P28068
NCBI GenInfo Identifier:	133160
NCBI Gene ID:	3109
NCBI Accession:	P28068.1
UniProt Related Accession:	P28068
Molecular Weight:
NCBI Full Name:	HLA class II histocompatibility antigen, DM beta chain
NCBI Synonym Full Names:	major histocompatibility complex, class II, DM beta
NCBI Official Symbol:	HLA-DMB
NCBI Official Synonym Symbols:	RING7; D6S221E
NCBI Protein Information:	HLA class II histocompatibility antigen, DM beta chain
UniProt Protein Name:	HLA class II histocompatibility antigen, DM beta chain
UniProt Synonym Protein Names:	MHC class II antigen DMB; Really interesting new gene 7 protein
Protein Family:	HLA class II histocompatibility antigen
UniProt Gene Name:	HLA-DMB
UniProt Entry Name:	DMB_HUMAN

	Western blot analysis of extracts of Raji cells, using HLA-DMB antibody (CAB8384) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human stomach using HLA-DMB antibody (CAB8384) at dilution of 1:100 (40x lens).