Antibody Name:	Anti-HLA-C Antibody
Antibody SKU:	CAB1013
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant protein of human HLA-C

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	LO2, HL-60, Jurkat, Mouse liver

Immunogen:	Recombinant protein of human HLA-C
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	3107
Uniprot:	P10321
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	41kDa
Observed MW:	41kDa

Synonyms:

HLA-C, D6S204, HLA-JY3, HLAC, HLC-C, MHC, PSORS1

Background:

HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described

UniProt Protein Function:	HLAC iso7: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: cell surface; early endosome membrane; endoplasmic reticulum; Golgi apparatus; Golgi membrane; MHC class I protein complex; phagocytic vesicle membrane; plasma membrane Molecular Function:antigen binding; peptide antigen binding Biological Process: antigen processing and presentation; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; regulation of immune response
NCBI Summary:	HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described [provided by RefSeq, Jul 2008]
UniProt Code:	P10321
NCBI GenInfo Identifier:	84028168
NCBI Gene ID:	3107
NCBI Accession:	P10321.3
UniProt Secondary Accession:	P10321,O78061, O78083, Q29631, Q29652, Q29867, Q29990 Q95463, Q95603, Q9MY31, Q9TQP9,
UniProt Related Accession:	P30499,P30501,P30504,P30505,Q07000,Q29865,Q29960,Q95604,P04222,P30508
Molecular Weight:	40,649 Da
NCBI Full Name:	HLA class I histocompatibility antigen, Cw-7 alpha chain
NCBI Synonym Full Names:	major histocompatibility complex, class I, C
NCBI Official Symbol:	HLA-C
NCBI Official Synonym Symbols:	MHC; HLAC; HLC-C; D6S204; PSORS1; HLA-JY3
NCBI Protein Information:	HLA class I histocompatibility antigen, Cw-1 alpha chain
UniProt Protein Name:	HLA class I histocompatibility antigen, Cw-7 alpha chain
UniProt Synonym Protein Names:	MHC class I antigen Cw*7
Protein Family:	HLA class I histocompatibility antigen
UniProt Gene Name:	HLA-C
UniProt Entry Name:	1C07_HUMAN

	Western blot analysis of extracts of various cell lines, using HLA-C antibody (CAB1013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of U2OS cells using HLA-C antibody (CAB1013) at dilution of 1:100. Blue: DAPI for nuclear staining.