Antibody Name:	Anti-HLA-B Antibody
Antibody SKU:	CAB1285
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HLA-B (NP_005505.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, Jurkat, HepG2, A-549, MCF7

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HLA-B (NP_005505.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLVM APRT VLLL LSAA LALT ETWA GSHS MRYF YTSV SRPG RGEP RFIS VGYV DDTQ FVRF DSDA ASPR EEPR APWI EQEG PEYW DRNT QIYK AQAQ TDRE
Gene ID:	3106
Uniprot:	P01889
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	40kDa
Observed MW:	40kDa

Synonyms:

HLA-B, AS, B-4901, HLAB, Bw-47, Bw-50, SPDA1

Background:

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.

UniProt Protein Function:	HLAB iso7: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.
UniProt Protein Details:	Protein type:Immunoglobulin superfamily; Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: cell surface; early endosome membrane; endoplasmic reticulum; Golgi apparatus; Golgi membrane; MHC class I protein complex; phagocytic vesicle membrane; plasma membrane Molecular Function:antigen binding; peptide antigen binding Biological Process: antigen processing and presentation; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; regulation of immune response
NCBI Summary:	HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	P01889
NCBI GenInfo Identifier:	122162
NCBI Gene ID:	3106
NCBI Accession:	P01889.3
UniProt Secondary Accession:	P01889,Q29638, Q29681, Q29854, Q29861, Q31613, Q5SRJ2 Q9GIX1, Q9TP95,
UniProt Related Accession:	P18463,P10319,P18464,P18465,P30460,P30461,P30462,P30466,P30685,P30475
Molecular Weight:	40,460 Da
NCBI Full Name:	HLA class I histocompatibility antigen, B-7 alpha chain
NCBI Synonym Full Names:	major histocompatibility complex, class I, B
NCBI Official Symbol:	HLA-B
NCBI Official Synonym Symbols:	AS; HLAB; Bw-47; Bw-50; SPDA1; B-4901; B-5001; HLA-Cw; HLA-B15; HLA-B39; HLA-B49; HLA-B50; HLA-B55; HLA-B59; HLA-B61; HEL-S-83; HLA-DRB1; HLA-B*45ZJ; HLA-B-3506; HLA-B-3905; HLA-B-5502; HLA-B-5602
NCBI Protein Information:	major histocompatibility complex, class I, B
UniProt Protein Name:	HLA class I histocompatibility antigen, B-7 alpha chain
UniProt Synonym Protein Names:	MHC class I antigen B*7
Protein Family:	HLA class I histocompatibility antigen
UniProt Gene Name:	HLA-B
UniProt Entry Name:	1B07_HUMAN

	Western blot analysis of extracts of various cell lines, using HLA-B antibody (CAB1285) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat spleen using HLA-B antibody (CAB1285) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal using HLA-B antibody (CAB1285) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using HLA-B antibody (CAB1285) at dilution of 1:100 (40x lens).