Cell Cycle Antibodies 1

Anti-Histone H2AX Antibody (CAB2082)

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SKU:
CAB2082
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-Histone H2AX Antibody
Antibody SKU:CAB2082
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Host Species:Rabbit
Immunogen:A synthetic peptide of human Histone H2AX.
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Positive Samples:293T, MCF7, HL-60, Rat heart
Immunogen:A synthetic peptide of human Histone H2AX.
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:3014
Uniprot:P16104
Cellular Location:Chromosome, Nucleus
Calculated MW:15kDa
Observed MW:15kDa
Synonyms:H2A.X, H2A/X, H2AX, Histone H2AX, H2AFX, histone H2AX, gamma H2A.X, GammaH2AX
Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
UniProt Protein Function:H2AX: a histone that replaces conventional H2A in a subset of nucleosomes. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Phosphorylated on S139 by ATM and DNA-PK in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Dephosphorylation of S139 by PP2A is required for DNA DSB repair. Apparently phosphorylated on Y143 by WSTF, determining the relative recruitment of either DNA repair or pro-apoptotic factors. H2AXpY142 favors the recruitment of pro-apoptotic factors APBB1 and JNK1. In contrast, dephosphorylation of pY143 by EYA phosphatases favors the recruitment of MDC1-containing DNA repair complexes to the tail of phosphorylated pS139. Monoubiquitination of K119 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'K63' linkages by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage.
UniProt Protein Details:

Protein type:DNA repair, damage; DNA-binding; Helicase

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: chromosome, telomeric region; condensed nuclear chromosome; male germ cell nucleus; nuclear chromatin; nucleoplasm; nucleosome; nucleus; replication fork; XY body

Molecular Function:damaged DNA binding; DNA binding; enzyme binding; histone binding; protein binding; protein heterodimerization activity

Biological Process: cerebral cortex development; chromatin silencing; DNA damage checkpoint; DNA repair; double-strand break repair; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; meiotic cell cycle; nucleosome assembly; positive regulation of DNA repair; response to DNA damage stimulus; response to ionizing radiation; spermatogenesis; viral reproduction

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]
UniProt Code:P16104
NCBI GenInfo Identifier:121992
NCBI Gene ID:3014
NCBI Accession:P16104.2
UniProt Secondary Accession:P16104,Q4ZGJ7, Q6IAS5,
UniProt Related Accession:P16104
Molecular Weight:15,145 Da
NCBI Full Name:Histone H2AX
NCBI Synonym Full Names:H2A histone family member X
NCBI Official Symbol:H2AFX  
NCBI Official Synonym Symbols:H2AX; H2A.X; H2A/X  
NCBI Protein Information:histone H2AX
UniProt Protein Name:Histone H2AX
UniProt Synonym Protein Names:Histone H2A.X
UniProt Gene Name:H2AFX  
UniProt Entry Name:H2AX_HUMAN
Anti-Histone H2AX Antibody (CAB2082)Western blot analysis of extracts of various cell lines, using Histone H2AX antibody (CAB2082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-Histone H2AX Antibody (CAB2082)Confocal immunofluorescence analysis of HeLa cells using Histone H2AX Polyclonal Antibody (CAB2082) at dilution of 1:200. Blue: DAPI for nuclear staining.
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