Antibody Name:	Anti-Histone H2A.Z Antibody [KO Validated]
Antibody SKU:	CAB6614
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human H2AFZ (NP_002097.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, MCF7, 22Rv1, PC-12, HepG2, Mouse testis, Mouse thymus, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human H2AFZ (NP_002097.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGG KAGK DSGK AKTK AVSR SQRA GLQF PVGR IHRH LKSR TTSH GRVG ATAA VYSA AILE YLTA EVLE LAGN ASKD LKVK RITP RHLQ LAIR GDEE LDSL IKAT IAGG GVIP HIHK SLIG KKGQ QKTV
Gene ID:	3015
Uniprot:	P0C0S5
Cellular Location:	Chromosome, Nucleus
Calculated MW:	13kDa
Observed MW:	15kDa

Synonyms:

H2AFZ, H2A.Z-1, H2A.z, H2A/z, H2AZ, histone H2A.Z

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality.

UniProt Protein Function:	H2AZ: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP. Belongs to the histone H2A family.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 4q23 Cellular Component: Barr body; nuclear heterochromatin; nucleus Molecular Function:chromatin DNA binding; nucleosomal DNA binding; protein binding Biological Process: chromatin silencing; positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]
UniProt Code:	P0C0S5
NCBI GenInfo Identifier:	83288408
NCBI Gene ID:	3015
NCBI Accession:	P0C0S5.2
UniProt Secondary Accession:	P0C0S5,P17317, Q6I9U0, B2RD56,
UniProt Related Accession:	P0C0S5
Molecular Weight:	14kDa
NCBI Full Name:	Histone H2A.Z
NCBI Synonym Full Names:	H2A histone family member Z
NCBI Official Symbol:	H2AFZ
NCBI Official Synonym Symbols:	H2AZ; H2A.z; H2A/z; H2A.Z-1
NCBI Protein Information:	histone H2A.Z
UniProt Protein Name:	Histone H2A.Z
UniProt Gene Name:	H2AFZ

	Western blot analysis of extracts of various cell lines, using H2AFZ antibody (CAB6614) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts from normal (control) and Histone H2A.Z Rabbit pAb knockout (KO) 293T cells, using Histone H2A.Z Rabbit pAb antibody (CAB6614) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded mouse kidney using Histone H2A.Z antibody (CAB6614) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using Histone H2A.Z antibody (CAB6614) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using Histone H2A.Z antibody (CAB6614) at dilution of 1:100 (40x lens).