Antibody Name:	Anti-Histone H1.0 Antibody
Antibody SKU:	CAB6918
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human H1F0 (NP_005309.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HepG2, Jurkat, HeLa, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human H1F0 (NP_005309.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTEN STSA PAAK PKRA KASK KSTD HPKY SDMI VAAI QAEK NRAG SSRQ SIQK YIKS HYKV GENA DSQI KLSI KRLV TTGV LKQT KGVG ASGS FRLA KSDE PKKS VAFK KTKK EIKK VATP KKAS KPKK AASK APTK KPKA TPVK KAKK KLAA TPKK AKKP KTVK AKPV KASK PKKA KPVK PKAK SSAK RAGK KK
Gene ID:	3005
Uniprot:	P07305
Cellular Location:	Chromosome, Nucleus
Calculated MW:	19kDa/20kDa
Observed MW:	36kDa

Synonyms:

H1F0, H10, H1FV, histone H1.0

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.

UniProt Protein Function:	H1F0: Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. Belongs to the histone H1/H5 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: nucleoplasm; Golgi apparatus; nuclear chromatin; nucleosome; nucleus; actin cytoskeleton Molecular Function:protein binding; chromatin DNA binding Biological Process: nucleosome assembly; apoptosis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis
NCBI Summary:	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. [provided by RefSeq, Jul 2008]
UniProt Code:	P07305
NCBI GenInfo Identifier:	121897
NCBI Gene ID:	3005
NCBI Accession:	P07305.3
UniProt Secondary Accession:	P07305,Q6FG88, Q8N6R3, B2R6I0, B4DRD6,
UniProt Related Accession:	P07305
Molecular Weight:	194
NCBI Full Name:	Histone H1.0
NCBI Synonym Full Names:	H1 histone family, member 0
NCBI Official Symbol:	H1F0
NCBI Official Synonym Symbols:	H10; H1FV
NCBI Protein Information:	histone H1.0; histone H1'; histone H1(0); H1.0, H1(0), H1-0
UniProt Protein Name:	Histone H1.0
UniProt Synonym Protein Names:	Histone H1'; Histone H1(0)Histone H1.0, N-terminally processed
Protein Family:	Histone
UniProt Gene Name:	H1F0
UniProt Entry Name:	H10_HUMAN

	Western blot analysis of extracts of various cell lines, using Histone H1.0 antibody (CAB6918) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U-2 OS cells using Histone H1.0 antibody (CAB6918) at dilution of 1:100. Blue: DAPI for nuclear staining.