Antibody Name:	Anti-Histone H1.0 Antibody [KO Validated]
Antibody SKU:	CAB3298
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human H1F0 (NP_005309.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human H1F0 (NP_005309.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTEN STSA PAAK PKRA KASK KSTD HPKY SDMI VAAI QAEK NRAG SSRQ SIQK YIKS HYKV GENA DSQI KLSI KRLV TTGV LKQT KGVG ASGS FRLA KSDE PKKS V
Gene ID:	3005
Uniprot:	P07305
Cellular Location:	Chromosome, Nucleus
Calculated MW:	19kDa/20kDa
Observed MW:	30KDa

Synonyms:

H1F0, H10, H1FV, histone H1.0

Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-independent histone that is a member of the histone H1 family.

UniProt Protein Function:	H1F0: Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. Belongs to the histone H1/H5 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: nucleoplasm; Golgi apparatus; nuclear chromatin; nucleosome; nucleus; actin cytoskeleton Molecular Function:protein binding; chromatin DNA binding Biological Process: nucleosome assembly; apoptosis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis
NCBI Summary:	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. [provided by RefSeq, Jul 2008]
UniProt Code:	P07305
NCBI GenInfo Identifier:	121897
NCBI Gene ID:	3005
NCBI Accession:	P07305.3
UniProt Secondary Accession:	P07305,Q6FG88, Q8N6R3, B2R6I0, B4DRD6,
UniProt Related Accession:	P07305
Molecular Weight:	194
NCBI Full Name:	Histone H1.0
NCBI Synonym Full Names:	H1 histone family, member 0
NCBI Official Symbol:	H1F0
NCBI Official Synonym Symbols:	H10; H1FV
NCBI Protein Information:	histone H1.0; histone H1'; histone H1(0); H1.0, H1(0), H1-0
UniProt Protein Name:	Histone H1.0
UniProt Synonym Protein Names:	Histone H1'; Histone H1(0)Histone H1.0, N-terminally processed
Protein Family:	Histone
UniProt Gene Name:	H1F0
UniProt Entry Name:	H10_HUMAN

	Western blot analysis of extracts of 293T cells, using Histone H1.0 antibody (CAB3298) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded mouse brain using Histone H1.0 antibody (CAB3298) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using Histone H1.0 antibody (CAB3298) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat liver using Histone H1.0 antibody (CAB3298) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using Histone H1.0 antibody (CAB3298) at dilution of 1:100. Blue: DAPI for nuclear staining.