Cell Biology Antibodies 10

Anti-HIRIP3 Antibody (CAB7014)

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SKU:
CAB7014
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-HIRIP3 Antibody
Antibody SKU:CAB7014
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 417-556 of human HIRIP3 (NP_003600.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60, HeLa, U-937, Jurkat, Mouse skeletal muscle, Mouse liver, Rat skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 417-556 of human HIRIP3 (NP_003600.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GRRG EDHP AVMR LKRY IRAC GAHR NYKK LLGS CCSH KERL SILR AELE ALGM KGTP SLGK CRAL KEQR EEAA EVAS LDVA NIIS GSGR PRRR TAWN PLGE AAPP GELY RRTL DSDE ERPR PAPP DWSH MRGI ISSD GESN
Gene ID:8479
Uniprot:Q9BW71
Cellular Location:Nucleus
Calculated MW:19kDa/27kDa/61kDa
Observed MW:62kDa
Synonyms:HIRIP3
Background:The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
UniProt Protein Function:HIRIP3: May play a role in chromatin function and histone metabolism via its interaction with HIRA and histones. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function:protein binding

Biological Process: chromatin assembly or disassembly

NCBI Summary:The HIRA protein shares sequence similarity with Hir1p and Hir2p, the two corepressors of histone gene transcription characterized in the yeast, Saccharomyces cerevisiae. The structural features of the HIRA protein suggest that it may function as part of a multiprotein complex. Several cDNAs encoding HIRA-interacting proteins, or HIRIPs, have been identified. In vitro, the protein encoded by this gene binds HIRA, as well as H2B and H3 core histones, indicating that a complex containing HIRA-HIRIP3 could function in some aspects of chromatin and histone metabolism. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
UniProt Code:Q9BW71
NCBI GenInfo Identifier:116242511
NCBI Gene ID:8479
NCBI Accession:Q9BW71.3
UniProt Secondary Accession:Q9BW71,O75707, O75708, H3BSR3,
UniProt Related Accession:Q9BW71
Molecular Weight:556
NCBI Full Name:HIRA-interacting protein 3
NCBI Synonym Full Names:HIRA interacting protein 3
NCBI Official Symbol:HIRIP3  
NCBI Protein Information:HIRA-interacting protein 3
UniProt Protein Name:HIRA-interacting protein 3
Protein Family:HIRA-interacting protein
UniProt Gene Name:HIRIP3  
UniProt Entry Name:HIRP3_HUMAN
Anti-HIRIP3 Antibody (CAB7014)Western blot analysis of extracts of various cell lines, using HIRIP3 antibody (CAB7014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-HIRIP3 Antibody (CAB7014)Immunohistochemistry of paraffin-embedded rat spleen using HIRIP3 antibody (CAB7014) at dilution of 1:100 (40x lens).
Anti-HIRIP3 Antibody (CAB7014)Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using HIRIP3 antibody (CAB7014) at dilution of 1:100 (40x lens).
Anti-HIRIP3 Antibody (CAB7014)Immunohistochemistry of paraffin-embedded mouse liver using HIRIP3 antibody (CAB7014) at dilution of 1:100 (40x lens).
Anti-HIRIP3 Antibody (CAB7014)Immunofluorescence analysis of U2OS cells using HIRIP3 antibody (CAB7014).
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