Epigenetics & Nuclear Signaling Antibodies 2
Anti-HIF1Beta/ARNT Antibody (CAB14705)
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- SKU:
- CAB14705
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-HIF1Beta/ARNT Antibody |
| Antibody SKU: | CAB14705 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human HIF1Beta/ARNT (NP_001659.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, Mouse brain, Rat brian |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human HIF1Beta/ARNT (NP_001659.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAT TANP EMTS DVPS LGPA IASG NSGP GIQG GGAI VQRA IKRR PGLD FDDD GEGN SKFL RCDD DQMS NDKE RFAR SDDE QSSA DKER LARE NHSE IERR RRNK MTAY ITEL SDMV PTCS ALAR KPDK LTIL RMAV SHMK SLRG TGNT STDG SYKP SFLT DQEL KHLI LEAA DGFL FIVS CETG RVVY VSDS VTPV LNQP QSEW FGST LYDQ VHPD DVDK LREQ LSTS ENAL TGRI LDLK TGTV KKEG QQSS MRMC MGSR |
| Gene ID: | 405 |
| Uniprot: | P27540 |
| Cellular Location: | Nucleus |
| Calculated MW: | 84kDa/85kDa/86kDa |
| Observed MW: | 87kDa |
| Synonyms: | ARNT, HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2 |
| Background: | This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia. |
| NCBI Summary: | This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013] |
| UniProt Code: | P27540 |
| NCBI GenInfo Identifier: | 114163 |
| NCBI Gene ID: | 405 |
| NCBI Accession: | P27540.1 |
| UniProt Secondary Accession: | P27540,Q59ED4, Q5QP39, Q8NDC7, B2R9H1, C4AMA1, F8WAP6 |
| UniProt Related Accession: | P27540 |
| Molecular Weight: | 86,366 Da |
| NCBI Full Name: | Aryl hydrocarbon receptor nuclear translocator |
| NCBI Synonym Full Names: | aryl hydrocarbon receptor nuclear translocator |
| NCBI Official Symbol: | ARNT |
| NCBI Official Synonym Symbols: | HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta |
| NCBI Protein Information: | aryl hydrocarbon receptor nuclear translocator |
| UniProt Protein Name: | Aryl hydrocarbon receptor nuclear translocator |
| UniProt Synonym Protein Names: | Class E basic helix-loop-helix protein 2; bHLHe2; Dioxin receptor, nuclear translocator; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-beta |
| Protein Family: | Aryl hydrocarbon receptor nuclear translocator |
| UniProt Gene Name: | ARNT |
 | Western blot analysis of extracts of various cell lines, using HIF1b/ARNT antibody (CAB14705) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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