Epigenetics & Nuclear Signaling Antibodies 3
Anti-HIC2 Antibody (CAB14313)
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- SKU:
- CAB14313
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-HIC2 Antibody |
| Antibody SKU: | CAB14313 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-420 of human HIC2 (NP_055909.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, Mouse brain, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-420 of human HIC2 (NP_055909.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LTAA SYLQ LPEL AALC RRKL KRAG KPFG SGRA GSTG MGRP PRSQ RLST ASVI QARY QGLV DGRK GAHA PQEL PQAK GSDD ELFL GGSN QDSV QGLG RAVC PAGG EAGL GGCS SSTN GSSG GCEQ ELGL DLSK KSPP LPPA TPGP HLTP DDAA QLSD SQHG SPPA ASAP PVAN SASY SELG GTPD EPMD LEGA EDNH LSLL EAPG GQPR KSLR HSTR KKEW GKKE PVAG SPFE RREA GPKG PCPG EEGE GVGD RVPN GILA SGAG PSGP YGEP PYPC KEEE ENGK DASE DSAQ SGSE GGSG H |
| Gene ID: | 23119 |
| Uniprot: | Q96JB3 |
| Cellular Location: | Nucleus |
| Calculated MW: | 64kDa/66kDa |
| Observed MW: | 75kDa |
| Synonyms: | HIC2, HRG22, ZBTB30, ZNF907 |
| Background: |
| UniProt Protein Function: | HIC2: Transcriptional repressor. Belongs to the krueppel C2H2-type zinc-finger protein family. Hic subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: nucleus Molecular Function:protein C-terminus binding |
| UniProt Code: | Q96JB3 |
| NCBI GenInfo Identifier: | 20454983 |
| NCBI Gene ID: | 23119 |
| NCBI Accession: | Q96JB3.2 |
| UniProt Secondary Accession: | Q96JB3,Q504T6, Q96KR3, Q9NSM9, Q9UPX9, |
| UniProt Related Accession: | Q96JB3 |
| Molecular Weight: | 64,228 Da |
| NCBI Full Name: | Hypermethylated in cancer 2 protein |
| NCBI Synonym Full Names: | hypermethylated in cancer 2 |
| NCBI Official Symbol: | HIC2 |
| NCBI Official Synonym Symbols: | HRG22; ZBTB30; ZNF907 |
| NCBI Protein Information: | hypermethylated in cancer 2 protein |
| UniProt Protein Name: | Hypermethylated in cancer 2 protein |
| UniProt Synonym Protein Names: | HIC1-related gene on chromosome 22 protein; Hic-3; Zinc finger and BTB domain-containing protein 30 |
| Protein Family: | Hypermethylated in cancer 2 protein |
| UniProt Gene Name: | HIC2 |
| UniProt Entry Name: | HIC2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HIC2 antibody (CAB14313) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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