Epigenetics & Nuclear Signaling Antibodies 4

Anti-HIC1 Antibody (CAB9651)

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SKU:
CAB9651
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-HIC1 Antibody
Antibody SKU:CAB9651
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 180-430 of human HIC1 (NP_001091672.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Rat
Positive Samples:NCI-H460, HT-29, DU145, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 180-430 of human HIC1 (NP_001091672.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AATP VIQA CYPS PVGP PPPP AAEP PSGP EAAV NTHC AELY ASGP GPAA ALCA SERR CSPL CGLD LSKK SPPG SAAP ERPL AERE LPPR PDSP PSAG PAAY KEPP LALP SLPP LPFQ KLEE AAPP SDPF RGGS GSPG PEPP GRPD GPSL LYRW MKHE PGLG SYGD ELGR ERGS PSER CEER GGDA AVSP GGPP LGLA PPPR YPGS LDGP GAGG DGDD YKSS SEET GSSE DPSP PGGH LEGY PCPH LAYG EPE
Gene ID:3090
Uniprot:Q14526
Cellular Location:Nucleus
Calculated MW:74kDa/76kDa
Observed MW:76kDa
Synonyms:HIC1, ZBTB29, ZNF901, hic-1
Background:This gene functions as a growth regulatory and tumor repressor gene. Hypermethylation or deletion of the region of this gene have been associated with tumors and the contiguous-gene syndrome, Miller-Dieker syndrome. Alternative splicing of this gene results in multiple transcript variants.
UniProt Protein Function:HIC1: Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1. The regulation of SIRT1 transcription in response to nutrient deprivation seems to involve CTBP1. In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells. Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF- responsive genes. Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex. Probably represses transcription from CXCR7, FGFBP1 and EFNA1. Belongs to the krueppel C2H2-type zinc-finger protein family. Hic subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor; Tumor suppressor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: chromatin; cytoplasm; nucleoplasm

Molecular Function:histone deacetylase binding; protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of Wnt receptor signaling pathway; positive regulation of DNA damage response, signal transduction by p53 class mediator; regulation of transcription, DNA-dependent

NCBI Summary:This gene functions as a growth regulatory and tumor repressor gene. Hypermethylation or deletion of the region of this gene have been associated with tumors and the contiguous-gene syndrome, Miller-Dieker syndrome. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2010]
UniProt Code:Q14526
NCBI GenInfo Identifier:296439502
NCBI Gene ID:3090
NCBI Accession:Q14526.5
UniProt Secondary Accession:Q14526,D3DTI4,
UniProt Related Accession:Q14526
Molecular Weight:74,514 Da
NCBI Full Name:Hypermethylated in cancer 1 protein
NCBI Synonym Full Names:hypermethylated in cancer 1
NCBI Official Symbol:HIC1  
NCBI Official Synonym Symbols:hic-1; ZBTB29; ZNF901  
NCBI Protein Information:hypermethylated in cancer 1 protein
UniProt Protein Name:Hypermethylated in cancer 1 protein
UniProt Synonym Protein Names:Zinc finger and BTB domain-containing protein 29
Protein Family:Hypermethylated in cancer 1 protein
UniProt Gene Name:HIC1  
UniProt Entry Name:HIC1_HUMAN
Anti-HIC1 Polyclonal Antibody (CAB9651)Western blot analysis of extracts of various cell lines, using HIC1 antibody (CAB9651) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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