KO Validated Antibodies 2

Anti-HIBADH Antibody (CAB19871)[KO Validated]

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SKU:
CAB19871
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-HIBADH Antibody [KO Validated]
Antibody SKU:CAB19871
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human HIBADH.
Application:WB IF
Recommended Dilution:WB 1:1000 - 1:3000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant protein of human HIBADH.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:11112
Uniprot:P31937
Cellular Location:Mitochondrion
Calculated MW:35kDa
Observed MW:32KDa
Anti-HIBADH Antibody [KO Validated] (CAB19871)Western blot analysis of extracts from normal (control) and HIBADH knockout (KO) HeLa cells, using HIBADH antibody (CAB19871) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:HIBADH, NS5ATP1
Background:This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde.
UniProt Protein Function:HIBADH: a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde. [provided by RefSeq, Nov 2011]
UniProt Protein Details:

Protein type:Mitochondrial; EC 1.1.1.31; Oxidoreductase; Amino Acid Metabolism - valine, leucine and isoleucine degradation

Chromosomal Location of Human Ortholog: 7p15.2

Cellular Component: mitochondrial matrix

Molecular Function:3-hydroxyisobutyrate dehydrogenase activity

Biological Process: branched chain family amino acid catabolic process; valine catabolic process

NCBI Summary:This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde. [provided by RefSeq, Nov 2011]
UniProt Code:P31937
NCBI GenInfo Identifier:12643395
NCBI Gene ID:11112
NCBI Accession:P31937.2
UniProt Secondary Accession:P31937,Q546Z2, Q9UDN3,
UniProt Related Accession:P31937
Molecular Weight:35,329 Da
NCBI Full Name:3-hydroxyisobutyrate dehydrogenase, mitochondrial
NCBI Synonym Full Names:3-hydroxyisobutyrate dehydrogenase
NCBI Official Symbol:HIBADH  
NCBI Official Synonym Symbols:NS5ATP1  
NCBI Protein Information:3-hydroxyisobutyrate dehydrogenase, mitochondrial
UniProt Protein Name:3-hydroxyisobutyrate dehydrogenase, mitochondrial
Protein Family:3-hydroxyisobutyrate dehydrogenase
UniProt Gene Name:HIBADH  
UniProt Entry Name:3HIDH_HUMAN
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