Cell Biology Antibodies 5

Anti-HHAT Antibody (CAB15171)

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SKU:
CAB15171
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-HHAT Antibody
Antibody SKU:CAB15171
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HHAT (NP_060664.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:A375, K-562, Mouse brain, Rat spleen
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HHAT (NP_060664.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLPR WELA LYLL ASLG FHFY SFYE VYKV SREH EEEL DQEF ELET DTLF GGLK KDAT DFEW SFWM EWGK QWLV WLLL GHMV VSQM ATLL ARKH RPWI LMLY
Gene ID:55733
Uniprot:Q5VTY9
Cellular Location:Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:9kDa/41kDa/49kDa/52kDa/56kDa/57kDa/60kDa
Observed MW:63kDa
Synonyms:HHAT, MART2, SKI1, Skn
Background:
NCBI Summary:'Skinny hedgehog' (SKI1) encodes an enzyme that acts within the secretory pathway to catalyze amino-terminal palmitoylation of 'hedgehog' (see MIM 600725).[supplied by OMIM, Jul 2002]
UniProt Code:Q5VTY9
NCBI GenInfo Identifier:170784863
NCBI Gene ID:55733
NCBI Accession:NP_001116306.1
UniProt Related Accession:Q5VTY9
Molecular Weight:9kDa; 41kDa; 49kDa; 52kDa; 56kDa; 57kDa; 60kDa
NCBI Full Name:protein-cysteine N-palmitoyltransferase HHAT isoform 1
NCBI Synonym Full Names:hedgehog acyltransferase
NCBI Official Symbol:HHAT  
NCBI Official Synonym Symbols:Skn; SKI1; MART2  
NCBI Protein Information:protein-cysteine N-palmitoyltransferase HHAT
UniProt Protein Name:Protein-cysteine N-palmitoyltransferase HHAT
UniProt Synonym Protein Names:Hedgehog acyltransferase; Melanoma antigen recognized by T-cells 2; MART-2; Skinny hedgehog protein 1
Protein Family:Protein-cysteine N-palmitoyltransferase
UniProt Gene Name:HHAT  
UniProt Entry Name:HHAT_HUMAN
Anti-HHAT Antibody (CAB15171)Western blot analysis of extracts of various cell lines, using HHAT antibody (CAB15171) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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