Cell Biology Antibodies 7
Anti-HGS Antibody (CAB1790)
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- SKU:
- CAB1790
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-HGS Antibody |
| Antibody SKU: | CAB1790 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 548-777 of human HGS (NP_004703.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | THP-1, OVCAR3, MCF7, HepG2, Mouse testis, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 548-777 of human HGS (NP_004703.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EQQK QTVQ MRAQ MPAF PLPY AQLQ AMPA AGGV LYQP SGPA SFPS TFSP AGSV EGSP MHGV YMSQ PAPA AGPY PSMP STAA DPSM VSAY MYPA GATG AQAA PQAQ AGPT ASPA YSSY QPTP TAGY QNVA SQAP QSLP AISQ PPQS STMG YMGS QSVS MGYQ PYNM QNLM TTLP SQDA SLPP QQPY IAGQ QPMY QQMA PSGG PPQQ QPPV AQQP QAQG PPAQ GSEA QLIS FD |
| Gene ID: | 9146 |
| Uniprot: | O14964 |
| Cellular Location: | Cytoplasm, Cytoplasmic side, Early endosome membrane, Endosome, Peripheral membrane protein, multivesicular body membrane |
| Calculated MW: | 76kDa/86kDa |
| Observed MW: | 110kDa |
| Synonyms: | HGS, HRS |
| Background: | The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. |
| UniProt Protein Function: | Hrs: a zinc finger protein which is rapidly tyrosine phosphorylated in cells stimulated with various growth factors. Hrs mRNA is expressed in all the human adult and fetal tissues tested. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 17q25 Cellular Component: cytoplasm; cytosol; early endosome; early endosome membrane; endosome; intracellular membrane-bound organelle Molecular Function:metal ion binding; protein binding; protein domain specific binding Biological Process: autophagy; endosome transport; epidermal growth factor receptor signaling pathway; membrane invagination; negative regulation of cell proliferation; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of JAK-STAT cascade; post-Golgi vesicle-mediated transport; protein targeting to lysosome; regulation of MAP kinase activity; regulation of protein catabolic process; signal transduction |
| NCBI Summary: | The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq, Dec 2010] |
| UniProt Code: | O14964 |
| NCBI GenInfo Identifier: | 71152119 |
| NCBI Gene ID: | 9146 |
| NCBI Accession: | O14964.1 |
| UniProt Secondary Accession: | O14964,Q9NR36, |
| UniProt Related Accession: | O14964 |
| Molecular Weight: | 76,376 Da |
| NCBI Full Name: | Hepatocyte growth factor-regulated tyrosine kinase substrate |
| NCBI Synonym Full Names: | hepatocyte growth factor-regulated tyrosine kinase substrate |
| NCBI Official Symbol: | HGS |
| NCBI Official Synonym Symbols: | HRS |
| NCBI Protein Information: | hepatocyte growth factor-regulated tyrosine kinase substrate |
| UniProt Protein Name: | Hepatocyte growth factor-regulated tyrosine kinase substrate |
| UniProt Synonym Protein Names: | Hrs |
| Protein Family: | Hepatocyte growth factor-regulated tyrosine kinase substrate |
| UniProt Gene Name: | HGS |
| UniProt Entry Name: | HGS_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HGS antibody (CAB1790) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U-2 OS cells using HGS Rabbit pAb (CAB1790) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining. |
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