Antibody Name:	Anti-HEY1 Antibody
Antibody SKU:	CAB16110
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human HEY1 (NP_001269780.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, 293T, Mouse heart, Mouse brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human HEY1 (NP_001269780.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IIEG LDAS DPLR VRLV SHLN NYAS QREA ASGA HAGL GHIP WGTV FGHH PHIA HPLL LPQN GHGN AGTT ASPT EPHH QGRL GSAH PEAP ALRA PPSG SLGP V
Gene ID:	23462
Uniprot:	Q9Y5J3
Cellular Location:	Nucleus
Calculated MW:	32kDa/33kDa
Observed MW:	35kDa

Synonyms:

HEY1, BHLHb31, CHF2, HERP2, HESR1, HRT-1, OAF1, hHRT1

Background:

This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	HEY1: Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5'- CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6. Belongs to the HEY family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 8q21 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:protein binding; protein dimerization activity; transcription factor activity; transcription factor binding Biological Process: angiogenesis; negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent
NCBI Summary:	This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y5J3
NCBI GenInfo Identifier:	105990528
NCBI Gene ID:	23462
NCBI Accession:	NP_036390.3
UniProt Secondary Accession:	Q9Y5J3,Q5TZS3, Q8NAM2, Q9NYP4, B2R883,
UniProt Related Accession:	Q9Y5J3
Molecular Weight:	33 kDa
NCBI Full Name:	hairy/enhancer-of-split related with YRPW motif protein 1 isoform a
NCBI Synonym Full Names:	hes related family bHLH transcription factor with YRPW motif 1
NCBI Official Symbol:	HEY1
NCBI Official Synonym Symbols:	CHF2; OAF1; HERP2; HESR1; HRT-1; hHRT1; BHLHb31
NCBI Protein Information:	hairy/enhancer-of-split related with YRPW motif protein 1
UniProt Protein Name:	Hairy/enhancer-of-split related with YRPW motif protein 1
UniProt Synonym Protein Names:	Cardiovascular helix-loop-helix factor 2; CHF-2; Class B basic helix-loop-helix protein 31; bHLHb31; HES-related repressor protein 1; Hairy and enhancer of split-related protein 1; HESR-1; Hairy-related transcription factor 1; HRT-1; hHRT1
Protein Family:	Hairy/enhancer-of-split related with YRPW motif protein
UniProt Gene Name:	HEY1
UniProt Entry Name:	HEY1_HUMAN

	Western blot analysis of extracts of various cell lines, using HEY1 antibody (CAB16110) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of A431 cells using HEY1 antibody (CAB16110) at dilution of 1:100. Blue: DAPI for nuclear staining.