Metabolism Antibodies 3
Anti-Hexokinase-3 Antibody (CAB8428)
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- SKU:
- CAB8428
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-Hexokinase-3 Antibody |
| Antibody SKU: | CAB8428 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human HK3 (NP_002106.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, HL-60, THP-1, NCI-H460, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human HK3 (NP_002106.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDSI GSSG LRQG EETL SCSE EGLP GPSD SSEL VQEC LQQF KVTR AQLQ QIQA SLLG SMEQ ALRG QASP APAV RMLP TYVG STPH GTEQ GDFV VLEL GATG ASLR VLWV TLTG IEGH RVEP RSQE FVIP QEVM LGAG QQLF DFAA HCLS EFLD AQPV NKQG LQLG FSFS FPCH QTGL DRST LISW TKGF RCSG VEGQ DVVQ LLRD AIRR QGAY NIDV VAVV NDTV GTMM GCEP GVRP CEVG LVVD TGTN ACYM EEAR HVAV LDED RGRV CV |
| Gene ID: | 3101 |
| Uniprot: | P52790 |
| Cellular Location: | |
| Calculated MW: | 99kDa |
| Observed MW: | 110kDa |
| Synonyms: | HK3, HKIII, HXK3 |
| Background: | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. |
| UniProt Protein Function: | HK3: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). |
| UniProt Protein Details: | Protein type:Transferase; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Enzyme, cellular metabolism; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose; EC 2.7.1.1; Kinase, other; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - galactose Chromosomal Location of Human Ortholog: 5q35.2 Cellular Component: cytosol Molecular Function:fructokinase activity; glucokinase activity; hexokinase activity; mannokinase activity Biological Process: cell glucose homeostasis; glucose transport; glycolysis |
| NCBI Summary: | Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq, Apr 2009] |
| UniProt Code: | P52790 |
| NCBI GenInfo Identifier: | 206729871 |
| NCBI Gene ID: | 3101 |
| NCBI Accession: | P52790.2 |
| UniProt Secondary Accession: | P52790,Q8N1E7, |
| UniProt Related Accession: | P52790 |
| Molecular Weight: | 99,025 Da |
| NCBI Full Name: | Hexokinase-3 |
| NCBI Synonym Full Names: | hexokinase 3 |
| NCBI Official Symbol: | HK3 |
| NCBI Official Synonym Symbols: | HXK3; HKIII |
| NCBI Protein Information: | hexokinase-3 |
| UniProt Protein Name: | Hexokinase-3 |
| UniProt Synonym Protein Names: | Hexokinase type III; HK III |
| Protein Family: | Probable histidine kinase |
| UniProt Gene Name: | HK3 |
| UniProt Entry Name: | HXK3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HK3 antibody (CAB8428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using HK3 antibody (CAB8428) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using HK3 antibody (CAB8428) at dilution of 1:100 (40x lens). |
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