Name: Anti-HEXIM1 Antibody (CAB5775)
Brand: Immunology Antibodies 2
SKU: CAB5775
Availability: OutOfStock

Antibody Name:	Anti-HEXIM1 Antibody
Antibody SKU:	CAB5775
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 210-359 of human HEXIM1 (NP_006451.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	SW480, HepG2, 293T, MCF7, HeLa, HL-60

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 210-359 of human HEXIM1 (NP_006451.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDDH DQEE PDLK TGLY SKRA AAKS DDTS DDDF MEEG GEED GGSD GMGG DGSE FLQR DFSE TYER YHTE SLQN MSKQ ELIK EYLE LEKC LSRM EDEN NRLR LESK RLGG DDAR VREL ELEL DRLR AENL QLLT ENEL HRQQ ERAP LSKF GD
Gene ID:	10614
Uniprot:	O94992
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	40kDa
Observed MW:	70kDa

Synonyms:	HEXIM1, CLP1, EDG1, HIS1, MAQ1
Background:	Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns.

UniProt Protein Function:	HEXIM1: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA. Up-regulated by HMBA (hexamethylene bisacetamide). Down-regulated by estrogen. Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues. Belongs to the HEXIM family.
UniProt Protein Details:	Protein type:Transcription factor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 17q21.31 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:cyclin-dependent protein kinase inhibitor activity; protein binding; snRNA binding Biological Process: heart development; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; transcription, DNA-dependent
NCBI Summary:	Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq, Jul 2008]
UniProt Code:	O94992
NCBI GenInfo Identifier:	74735573
NCBI Gene ID:	10614
NCBI Accession:	O94992.1
UniProt Secondary Accession:	O94992,B2R8Y5,
UniProt Related Accession:	O94992
Molecular Weight:	40,623 Da
NCBI Full Name:	Protein HEXIM1
NCBI Synonym Full Names:	hexamethylene bis-acetamide inducible 1
NCBI Official Symbol:	HEXIM1
NCBI Official Synonym Symbols:	CLP1; EDG1; HIS1; MAQ1
NCBI Protein Information:	protein HEXIM1
UniProt Protein Name:	Protein HEXIM1
UniProt Synonym Protein Names:	Cardiac lineage protein 1; Estrogen down-regulated gene 1 protein; Hexamethylene bis-acetamide-inducible protein 1; Menage a quatre protein 1
Protein Family:	Protein
UniProt Gene Name:	HEXIM1
UniProt Entry Name:	HEXI1_HUMAN

	Western blot analysis of extracts of various cell lines, using HEXIM1 antibody (CAB5775) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat brain using HEXIM1 Antibody (CAB5775) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using HEXIM1 Antibody (CAB5775) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon cancer using HEXIM1 Antibody (CAB5775) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using HEXIM1 antibody (CAB5775). Blue: DAPI for nuclear staining.