Epigenetics & Nuclear Signaling Antibodies 1
Anti-HES1 Antibody (CAB11718)
- SKU:
- CAB11718
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-HES1 Antibody |
| Antibody SKU: | CAB11718 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 151-280 of human HES1 (NP_005515.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, Mouse intestine |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 151-280 of human HES1 (NP_005515.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NAMT YPGQ PHPA LQAP PPPP PGPG GPQH APFA PPPP LVPI PGGA APPP GGAP CKLG SQAG EAAK VFGG FQVV PAPD GQFA FLIP NGAF AHSG PVIP VYTS NSGT SVGP NAVS PSSG PSLT ADSM WRPW RN |
| Gene ID: | 3280 |
| Uniprot: | Q14469 |
| Cellular Location: | Nucleus |
| Calculated MW: | 29kDa |
| Observed MW: | 25kDa |
| Synonyms: | HES1, HES-1, HHL, HRY, bHLHb39 |
| Background: | This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. |
| UniProt Protein Function: | HES1: Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Transcription factor; Cell development/differentiation Chromosomal Location of Human Ortholog: 3q28-q29 Cellular Component: nucleoplasm; nucleus Molecular Function:DNA binding; histone deacetylase binding; protein binding; protein homodimerization activity; sequence-specific DNA binding; transcription factor activity Biological Process: artery morphogenesis; negative regulation of oligodendrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nervous system development; Notch signaling pathway; positive regulation of astrocyte differentiation; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of JAK-STAT cascade; positive regulation of mitotic cell cycle, embryonic; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; protein complex assembly; radial glial cell differentiation in the forebrain; STAT protein nuclear translocation; thymus development |
| NCBI Summary: | This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q14469 |
| NCBI GenInfo Identifier: | 3913825 |
| NCBI Gene ID: | 3280 |
| NCBI Accession: | Q14469.1 |
| UniProt Secondary Accession: | Q14469,Q6FHB2, |
| UniProt Related Accession: | Q14469 |
| Molecular Weight: | 29,541 Da |
| NCBI Full Name: | Transcription factor HES-1 |
| NCBI Synonym Full Names: | hes family bHLH transcription factor 1 |
| NCBI Official Symbol: | HES1 |
| NCBI Official Synonym Symbols: | HHL; HRY; HES-1; bHLHb39 |
| NCBI Protein Information: | transcription factor HES-1 |
| UniProt Protein Name: | Transcription factor HES-1 |
| UniProt Synonym Protein Names: | Class B basic helix-loop-helix protein 39; bHLHb39; Hairy and enhancer of split 1; Hairy homolog; Hairy-like protein; hHL |
| Protein Family: | Protein |
| UniProt Gene Name: | HES1 |
| UniProt Entry Name: | HES1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HES1 antibody (CAB11718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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