Immunology Antibodies 1

Anti-HERC5 Antibody (CAB14889)

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SKU:
CAB14889
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-HERC5 Antibody
Antibody SKU:CAB14889
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human HERC5 (NP_057407.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, OVCAR3, HT-1080, Mouse kidney, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human HERC5 (NP_057407.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ELIM IAGG NQSI LLWI KKEN SYVN LKRT IPTL NEGT VKRW IADV ETKR WQST KREI QEIF SSPA CLTG SFLR KRRT TEMM PVYL DLNK ARNI FKEL TQKD WITN MITT CLKD NLLK RLPF HSPP QEAL EIFF LLPE CPMM HISN NWES LVVP FAKV VCKM SDQS SLVL EEYW ATLQ ESTF SKLV QMFK TAVI CQLD YWDE SAEE NGNV QALL EMLK KLHR VNQV KCQL PESI FQVD ELLH RLNF FVEV CRRY LWKM TVDA SENV QCCV IFSH FPFI FNNL SKIK LLHT DTLL KIES KKHK AYLR SAAI EEER ESEF ALRP TFDL TVRR NHLI EDVL NQLS Q
Gene ID:51191
Uniprot:Q9UII4
Cellular Location:Cytoplasm, perinuclear region
Calculated MW:116kDa
Observed MW:117kDa
Synonyms:HERC5, CEB1, CEBP1
Background:This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4.
UniProt Protein Function:HERC5: Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Makes part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant- negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.
UniProt Protein Details:

Protein type:Ligase; EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: cytosol; perinuclear region of cytoplasm

Molecular Function:ISG15 conjugating enzyme activity; ligase activity; protein binding; ubiquitin-protein ligase activity

Biological Process: cytokine and chemokine mediated signaling pathway; defense response to virus; innate immune response; ISG15-protein conjugation; negative regulation of interferon type I production; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of cyclin-dependent protein kinase activity; regulation of defense response to virus

NCBI Summary:This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UII4
NCBI GenInfo Identifier:296434523
NCBI Gene ID:51191
NCBI Accession:Q9UII4.2
UniProt Secondary Accession:Q9UII4,Q69G20, B2RTQ1,
UniProt Related Accession:Q9UII4
Molecular Weight:116,852 Da
NCBI Full Name:E3 ISG15--protein ligase HERC5
NCBI Synonym Full Names:HECT and RLD domain containing E3 ubiquitin protein ligase 5
NCBI Official Symbol:HERC5  
NCBI Official Synonym Symbols:CEB1; CEBP1  
NCBI Protein Information:E3 ISG15--protein ligase HERC5
UniProt Protein Name:E3 ISG15--protein ligase HERC5
UniProt Synonym Protein Names:Cyclin-E-binding protein 1; HECT domain and RCC1-like domain-containing protein 5
Protein Family:E3 ISG15--protein ligase
UniProt Gene Name:HERC5  
UniProt Entry Name:HERC5_HUMAN
Anti-HERC5 Antibody (CAB14889)Western blot analysis of extracts of various cell lines, using HERC5 antibody (CAB14889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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