KO Validated Antibodies 1

Anti-HERC4 Antibody (CAB18049)[KO Validated]

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SKU:
CAB18049
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-HERC4 Antibody [KO Validated]
Antibody SKU:CAB18049
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human HERC4 (NP_056416.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-700 of human HERC4 (NP_056416.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, pH7.3.
Isotype:IgG
Sequence:WTVN EALI QKWL SYPS GRFP VEIA NEID GTFS SSGC LNGS FLAV SNDD HYRT GTRF SGVD MNAA RLLF HKLI QPDH PQIS QQVA ASLE KNLI PKLT SSLP DVEA LRFY LTLP ECPL MSDS NNFT TIAI PFGT ALVN LEKA PLKV LENW WSVL EPPL FLKI VELF KEVV VHLL KLYK IGIP PSER RIFN SFLH TALK VLEI LHRV NEKM GQII QYDK FYIH EVQE LIDI RNDY INWV QQQA YGML ADIP VTIC TYPF VFDA QAKT TLLQ TDAV LQMQ MAID QAHR QNVS SLFL PVIE SVNP C
Gene ID:26091
Uniprot:Q5GLZ8
Cellular Location:Cytoplasm, cytosol
Calculated MW:12kDa/13kDa/106kDa/109kDa/117kDa/118kDa
Observed MW:118kDa
Anti-HERC4 Antibody [KO Validated] (CAB18049)Western blot analysis of extracts from normal (control) and HERC4 knockout (KO) HeLa cells, using HERC4 antibody (CAB18049) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1min.
Synonyms:HERC4
Background:
UniProt Protein Function:HERC4: Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 6.3.2.19; Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 10q21.3

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function:ubiquitin-protein ligase activity; ligase activity

Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process; spermatogenesis; cell differentiation

NCBI Summary:HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008]
UniProt Code:Q5GLZ8
NCBI GenInfo Identifier:74707832
NCBI Gene ID:26091
NCBI Accession:Q5GLZ8.1
UniProt Secondary Accession:Q5GLZ8,Q5GC98, Q5GC99, Q5GCA0, Q8IXP9, Q9HCH9,
UniProt Related Accession:Q5GLZ8
Molecular Weight:1057
NCBI Full Name:Probable E3 ubiquitin-protein ligase HERC4
NCBI Synonym Full Names:HECT and RLD domain containing E3 ubiquitin protein ligase 4
NCBI Official Symbol:HERC4  
NCBI Protein Information:probable E3 ubiquitin-protein ligase HERC4; hect domain and RLD 4; HECT domain and RCC1-like domain-containing protein 4
UniProt Protein Name:Probable E3 ubiquitin-protein ligase HERC4
UniProt Synonym Protein Names:HECT domain and RCC1-like domain-containing protein 4
Protein Family:Probable E3 ubiquitin-protein ligase
UniProt Gene Name:HERC4  
UniProt Entry Name:HERC4_HUMAN
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