Epigenetics & Nuclear Signaling Antibodies 1
Anti-HEMK1 Antibody (CAB12651)
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- SKU:
- CAB12651
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-HEMK1 Antibody |
| Antibody SKU: | CAB12651 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-338 of human HEMK1 (NP_057257.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:3000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-338 of human HEMK1 (NP_057257.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MELW GRML WALL SGPG RRGS TRGW AFSS WQPQ PPLA GLSS AIEL VSHW TGVF EKRG IPEA RESS EYIV AHVL GAKT FQSL RPAL WTQP LTSQ QLQC IREL SSRR LQRM PVQY ILGE WDFQ GLSL RMVP PVFI PRPE TEEL VEWV LEEV AQRS HAVG SPGS PLIL EVGC GSGA ISLS LLSQ LPQS RVIA VDKR EAAI SLTH ENAQ RLRL QDRI WIIH LDMT SERS WTHL PWGP MDLI VSNP PYVF HQDM EQLA PEIR SYED PAAL DGGE EGMD IITH ILAL APRL LKDS GSIF LEVD PRHP ELVS SWLQ SRPD LYLN LVAV RRDF CGRP RFLH IRRS GP |
| Gene ID: | 51409 |
| Uniprot: | Q9Y5R4 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 38kDa |
| Observed MW: | 38kDa |
| Synonyms: | HEMK1, HEMK, MTQ1 |
| Background: |
| UniProt Protein Function: | HEMK1: N5-glutamine methyltransferase responsible for the methylation of the GGQ triplet of the mitochondrial translation release factor MTRF1L. Belongs to the protein N5-glutamine methyltransferase family. |
| UniProt Protein Details: | Protein type:EC 2.1.1.-; Methyltransferase; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - histidine; Lipid Metabolism - androgen and estrogen; Other Amino Acids Metabolism - selenoamino acid Chromosomal Location of Human Ortholog: 3p21.3 Molecular Function:protein binding |
| UniProt Code: | Q9Y5R4 |
| NCBI GenInfo Identifier: | 18203634 |
| NCBI Gene ID: | 51409 |
| NCBI Accession: | Q9Y5R4.1 |
| UniProt Related Accession: | Q9Y5R4 |
| Molecular Weight: | 38,231 Da |
| NCBI Full Name: | HemK methyltransferase family member 1 |
| NCBI Synonym Full Names: | HemK methyltransferase family member 1 |
| NCBI Official Symbol: | HEMK1 |
| NCBI Official Synonym Symbols: | HEMK; MTQ1 |
| NCBI Protein Information: | hemK methyltransferase family member 1 |
| UniProt Protein Name: | HemK methyltransferase family member 1 |
| UniProt Synonym Protein Names: | M.HsaHemKP |
| Protein Family: | HemK methyltransferase family |
| UniProt Gene Name: | HEMK1 |
| UniProt Entry Name: | HEMK1_HUMAN |
 | Western blot analysis of extracts of mouse kidney, using HEMK1 antibody (CAB12651) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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